9t4r: Difference between revisions

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'''Unreleased structure'''


The entry 9t4r is ON HOLD  until Paper Publication
==Staphylococcus aureus 70S initiation complex with a natural mRNA==
<StructureSection load='9t4r' size='340' side='right'caption='[[9t4r]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9t4r]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9T4R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9T4R FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9t4r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9t4r OCA], [https://pdbe.org/9t4r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9t4r RCSB], [https://www.ebi.ac.uk/pdbsum/9t4r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9t4r ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Regulation of translation initiation is central to bacterial adaptation, but species-specific mechanisms remain poorly understood. We present high-resolution mapping of translation start sites in Staphylococcus aureus, revealing distinct features of initiation alongside numerous unannotated small ORFs. Our analysis, combined with cryo-EM of a native mRNA-ribosome complex, shows that S. aureus relies on extended, start codon proximal Shine-Dalgarno (SD) interactions, creating specificity against phylogenetically distant bacteria. Several natural S. aureus initiation sites are not correctly decoded by E. coli ribosomes. We identify new and conserved non-canonical start codons, whose regulatory initiation sites contain these characteristic extended SD sequence motifs. Finally, we characterize a novel example of uORF-mediated translational control in S. aureus, demonstrating that translation of a small leader peptide modulates expression of a key biofilm regulator. The described mechanism involves codon rarity, ribosome pausing, and arginine availability, linking nutrient sensing to biofilm formation in this major human pathogen.


Authors:  
Extended Shine-Dalgarno motifs govern translation initiation in Staphylococcus aureus.,Kohl MP, Bahena-Ceron R, Chane-Woon-Ming B, Kompatscher M, Erlacher MD, Barchet C, von Loeffelholz O, Romby P, Klaholz BP, Marzi S Nat Commun. 2026 Feb 12. doi: 10.1038/s41467-026-69079-8. PMID:41680142<ref>PMID:41680142</ref>


Description:  
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
<div class="pdbe-citations 9t4r" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Staphylococcus aureus]]
[[Category: Bahena Ceron R]]
[[Category: Klaholz B]]
[[Category: Marzi S]]
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