9ig8: Difference between revisions

Latest revision as of 07:42, 4 March 2026

Apo-MtbKu , not bound to DNA

Structural highlights

9ig8 is a 2 chain structure with sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 4.41Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

KU_MYCTU With LigD forms a non-homologous end joining (NHEJ) repair enzyme. Binds linear dsDNA with 5'- and 3'-overhangs but not closed circular dsDNA nor ssDNA. One dimer binds for every 30 bp. Recruits and stimulates the ligase activity of LigD but not of T4 ligase or a human ligase complex (LIG4/XRCC4). Attenuates the 3'- to 5'-exonuclease activity of LigD. Stimulates the template-directed addition of dNTPs by LigD on 5'-overhangs and nuclease activity on 3'-overhangs.^[1] ^[2] ^[3] ^[4]

References

↑ Weller GR, Kysela B, Roy R, Tonkin LM, Scanlan E, Della M, Devine SK, Day JP, Wilkinson A, d'Adda di Fagagna F, Devine KM, Bowater RP, Jeggo PA, Jackson SP, Doherty AJ. Identification of a DNA nonhomologous end-joining complex in bacteria. Science. 2002 Sep 6;297(5587):1686-9. PMID:12215643 doi:10.1126/science.1074584
↑ Della M, Palmbos PL, Tseng HM, Tonkin LM, Daley JM, Topper LM, Pitcher RS, Tomkinson AE, Wilson TE, Doherty AJ. Mycobacterial Ku and ligase proteins constitute a two-component NHEJ repair machine. Science. 2004 Oct 22;306(5696):683-5. doi: 10.1126/science.1099824. PMID:15499016 doi:https://dx.doi.org/10.1126/science.1099824
↑ Gong C, Bongiorno P, Martins A, Stephanou NC, Zhu H, Shuman S, Glickman MS. Mechanism of nonhomologous end-joining in mycobacteria: a low-fidelity repair system driven by Ku, ligase D and ligase C. Nat Struct Mol Biol. 2005 Apr;12(4):304-12. PMID:15778718 doi:10.1038/nsmb915
↑ Pitcher RS, Tonkin LM, Green AJ, Doherty AJ. Domain structure of a NHEJ DNA repair ligase from Mycobacterium tuberculosis. J Mol Biol. 2005 Aug 19;351(3):531-44. PMID:16023671 doi:10.1016/j.jmb.2005.06.038

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OCA

[1] Weller GR, Kysela B, Roy R, Tonkin LM, Scanlan E, Della M, Devine SK, Day JP, Wilkinson A, d'Adda di Fagagna F, Devine KM, Bowater RP, Jeggo PA, Jackson SP, Doherty AJ. Identification of a DNA nonhomologous end-joining complex in bacteria. Science. 2002 Sep 6;297(5587):1686-9. PMID:12215643 doi:10.1126/science.1074584

[2] Della M, Palmbos PL, Tseng HM, Tonkin LM, Daley JM, Topper LM, Pitcher RS, Tomkinson AE, Wilson TE, Doherty AJ. Mycobacterial Ku and ligase proteins constitute a two-component NHEJ repair machine. Science. 2004 Oct 22;306(5696):683-5. doi: 10.1126/science.1099824. PMID:15499016 doi:https://dx.doi.org/10.1126/science.1099824

[3] Gong C, Bongiorno P, Martins A, Stephanou NC, Zhu H, Shuman S, Glickman MS. Mechanism of nonhomologous end-joining in mycobacteria: a low-fidelity repair system driven by Ku, ligase D and ligase C. Nat Struct Mol Biol. 2005 Apr;12(4):304-12. PMID:15778718 doi:10.1038/nsmb915

[4] Pitcher RS, Tonkin LM, Green AJ, Doherty AJ. Domain structure of a NHEJ DNA repair ligase from Mycobacterium tuberculosis. J Mol Biol. 2005 Aug 19;351(3):531-44. PMID:16023671 doi:10.1016/j.jmb.2005.06.038

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[2]

[3]

[4]

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 9ig8 is ON HOLD  until Paper Publication
+==Apo-MtbKu , not bound to DNA==
+<StructureSection load='9ig8' size='340' side='right'caption='[[9ig8]], [[Resolution|resolution]] 4.41&Aring;' scene=''>
-Authors:
+== Structural highlights ==
+<table><tr><td colspan='2'>[[9ig8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9IG8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9IG8 FirstGlance]. <br>
-Description:
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.41&#8491;</td></tr>
-[[Category: Unreleased Structures]]
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9ig8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9ig8 OCA], [https://pdbe.org/9ig8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9ig8 RCSB], [https://www.ebi.ac.uk/pdbsum/9ig8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9ig8 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/KU_MYCTU KU_MYCTU] With LigD forms a non-homologous end joining (NHEJ) repair enzyme. Binds linear dsDNA with 5'- and 3'-overhangs but not closed circular dsDNA nor ssDNA. One dimer binds for every 30 bp. Recruits and stimulates the ligase activity of LigD but not of T4 ligase or a human ligase complex (LIG4/XRCC4). Attenuates the 3'- to 5'-exonuclease activity of LigD. Stimulates the template-directed addition of dNTPs by LigD on 5'-overhangs and nuclease activity on 3'-overhangs.<ref>PMID:12215643</ref> <ref>PMID:15499016</ref> <ref>PMID:15778718</ref> <ref>PMID:16023671</ref>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Mycobacterium tuberculosis]]
+[[Category: Chaplin AK]]
+[[Category: Zahid S]]

9ig8: Difference between revisions

Latest revision as of 07:42, 4 March 2026

Apo-MtbKu , not bound to DNA

Structural highlights

Function

References

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