9kq0: Difference between revisions

Latest revision as of 07:44, 4 March 2026

Structure of TolQRA complex at pH 8.0 from E.coli

Structural highlights

9kq0 is a 12 chain structure with sequence from Escherichia coli K-12 and Saccharomyces cerevisiae S288C. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.6Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TOLQ_ECOLI Part of the Tol-Pal system, which plays a role in outer membrane invagination during cell division and is important for maintaining outer membrane integrity (PubMed:1683466, PubMed:17233825). Required, with TolR, for the proton motive force-dependent activation of TolA and for TolA-Pal interaction (PubMed:11722743). The Tol-Pal system is also required for polar localization of chemoreceptors clusters (PubMed:24720726). The system also appears to be required for the activity of several outer membrane-localized enzymes with cell wall remodeling activity (PubMed:32152098). Is involved in the uptake of group A colicins (colicins A, E1, E2, E3, and K) and in the uptake of filamentous phage DNA (PubMed:1683466, PubMed:3294803).^[1] ^[2] ^[3] ^[4] ^[5] ^[6] ^[7]

References

↑ Cascales E, Lloubès R, Sturgis JN. The TolQ-TolR proteins energize TolA and share homologies with the flagellar motor proteins MotA-MotB. Mol Microbiol. 2001 Nov;42(3):795-807. PMID:11722743 doi:10.1046/j.1365-2958.2001.02673.x
↑ Webster RE. The tol gene products and the import of macromolecules into Escherichia coli. Mol Microbiol. 1991 May;5(5):1005-11. PMID:1683466 doi:10.1111/j.1365-2958.1991.tb01873.x
↑ Gerding MA, Ogata Y, Pecora ND, Niki H, de Boer PA. The trans-envelope Tol-Pal complex is part of the cell division machinery and required for proper outer-membrane invagination during cell constriction in E. coli. Mol Microbiol. 2007 Feb;63(4):1008-25. PMID:17233825 doi:10.1111/j.1365-2958.2006.05571.x
↑ Santos TM, Lin TY, Rajendran M, Anderson SM, Weibel DB. Polar localization of Escherichia coli chemoreceptors requires an intact Tol-Pal complex. Mol Microbiol. 2014 Jun;92(5):985-1004. PMID:24720726 doi:10.1111/mmi.12609
↑ Yakhnina AA, Bernhardt TG. The Tol-Pal system is required for peptidoglycan-cleaving enzymes to complete bacterial cell division. Proc Natl Acad Sci U S A. 2020 Mar 24;117(12):6777-6783. PMID:32152098 doi:10.1073/pnas.1919267117
↑ Sun TP, Webster RE. Nucleotide sequence of a gene cluster involved in entry of E colicins and single-stranded DNA of infecting filamentous bacteriophages into Escherichia coli. J Bacteriol. 1987 Jun;169(6):2667-74. PMID:3294803 doi:10.1128/jb.169.6.2667-2674.1987
↑ Webster RE. The tol gene products and the import of macromolecules into Escherichia coli. Mol Microbiol. 1991 May;5(5):1005-11. PMID:1683466 doi:10.1111/j.1365-2958.1991.tb01873.x

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Cascales E, Lloubès R, Sturgis JN. The TolQ-TolR proteins energize TolA and share homologies with the flagellar motor proteins MotA-MotB. Mol Microbiol. 2001 Nov;42(3):795-807. PMID:11722743 doi:10.1046/j.1365-2958.2001.02673.x

[2] Webster RE. The tol gene products and the import of macromolecules into Escherichia coli. Mol Microbiol. 1991 May;5(5):1005-11. PMID:1683466 doi:10.1111/j.1365-2958.1991.tb01873.x

[3] Gerding MA, Ogata Y, Pecora ND, Niki H, de Boer PA. The trans-envelope Tol-Pal complex is part of the cell division machinery and required for proper outer-membrane invagination during cell constriction in E. coli. Mol Microbiol. 2007 Feb;63(4):1008-25. PMID:17233825 doi:10.1111/j.1365-2958.2006.05571.x

[4] Santos TM, Lin TY, Rajendran M, Anderson SM, Weibel DB. Polar localization of Escherichia coli chemoreceptors requires an intact Tol-Pal complex. Mol Microbiol. 2014 Jun;92(5):985-1004. PMID:24720726 doi:10.1111/mmi.12609

[5] Yakhnina AA, Bernhardt TG. The Tol-Pal system is required for peptidoglycan-cleaving enzymes to complete bacterial cell division. Proc Natl Acad Sci U S A. 2020 Mar 24;117(12):6777-6783. PMID:32152098 doi:10.1073/pnas.1919267117

[6] Sun TP, Webster RE. Nucleotide sequence of a gene cluster involved in entry of E colicins and single-stranded DNA of infecting filamentous bacteriophages into Escherichia coli. J Bacteriol. 1987 Jun;169(6):2667-74. PMID:3294803 doi:10.1128/jb.169.6.2667-2674.1987

[7] Webster RE. The tol gene products and the import of macromolecules into Escherichia coli. Mol Microbiol. 1991 May;5(5):1005-11. PMID:1683466 doi:10.1111/j.1365-2958.1991.tb01873.x

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 9kq0 is ON HOLD  until Paper Publication
+==Structure of TolQRA complex at pH 8.0 from E.coli==
+<StructureSection load='9kq0' size='340' side='right'caption='[[9kq0]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
-Authors:
+== Structural highlights ==
+<table><tr><td colspan='2'>[[9kq0]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9KQ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9KQ0 FirstGlance]. <br>
-Description:
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.6&#8491;</td></tr>
-[[Category: Unreleased Structures]]
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9kq0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9kq0 OCA], [https://pdbe.org/9kq0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9kq0 RCSB], [https://www.ebi.ac.uk/pdbsum/9kq0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9kq0 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/TOLQ_ECOLI TOLQ_ECOLI] Part of the Tol-Pal system, which plays a role in outer membrane invagination during cell division and is important for maintaining outer membrane integrity (PubMed:1683466, PubMed:17233825). Required, with TolR, for the proton motive force-dependent activation of TolA and for TolA-Pal interaction (PubMed:11722743). The Tol-Pal system is also required for polar localization of chemoreceptors clusters (PubMed:24720726). The system also appears to be required for the activity of several outer membrane-localized enzymes with cell wall remodeling activity (PubMed:32152098). Is involved in the uptake of group A colicins (colicins A, E1, E2, E3, and K) and in the uptake of filamentous phage DNA (PubMed:1683466, PubMed:3294803).<ref>PMID:11722743</ref> <ref>PMID:1683466</ref> <ref>PMID:17233825</ref> <ref>PMID:24720726</ref> <ref>PMID:32152098</ref> <ref>PMID:3294803</ref> <ref>PMID:1683466</ref>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Escherichia coli K-12]]
+[[Category: Large Structures]]
+[[Category: Saccharomyces cerevisiae S288C]]
+[[Category: Dong C]]
+[[Category: Zhang Z]]

9kq0: Difference between revisions

Latest revision as of 07:44, 4 March 2026

Structure of TolQRA complex at pH 8.0 from E.coli

Structural highlights

Function

References

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