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<StructureSection load='6sf7' size='340' side='right'caption='[[6sf7]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
<StructureSection load='6sf7' size='340' side='right'caption='[[6sf7]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6sf7]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staa3 Staa3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SF7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SF7 FirstGlance]. <br>
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SF7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SF7 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">splF, SAUSA300_1753 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=367830 STAA3])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6sf7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sf7 OCA], [https://pdbe.org/6sf7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6sf7 RCSB], [https://www.ebi.ac.uk/pdbsum/6sf7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6sf7 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6sf7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sf7 OCA], [https://pdbe.org/6sf7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6sf7 RCSB], [https://www.ebi.ac.uk/pdbsum/6sf7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6sf7 ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Accumulating evidence suggests that six proteases encoded in the spl operon of a dangerous human pathogen, Staphylococcus aureus, may play a role in virulence. Interestingly, SplA, B, D, and E have complementary substrate specificities while SplF remains to be characterized in this regard. Here, we describe the prerequisites of a heterologous expression system for active SplF protease and characterize the enzyme in terms of substrate specificity and its structural determinants. Substrate specificity of SplF is comprehensively profiled using combinatorial libraries of peptide substrates demonstrating strict preference for long aliphatic sidechains at the P1 subsite and significant selectivity for aromatic residues at P3. The crystal structure of SplF was provided at 1.7 A resolution to define the structural basis of substrate specificity of SplF. The obtained results were compared and contrasted with the characteristics of other Spl proteases determined to date to conclude that the spl operon encodes a unique extracellular proteolytic system.
Structural Determinants of Substrate Specificity of SplF Protease from Staphylococcus aureus.,Stach N, Karim A, Golik P, Kitel R, Pustelny K, Gruba N, Groborz K, Jankowska U, Kedracka-Krok S, Wladyka B, Drag M, Lesner A, Dubin G Int J Mol Sci. 2021 Feb 23;22(4). pii: ijms22042220. doi: 10.3390/ijms22042220. PMID:33672341<ref>PMID:33672341</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6sf7" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Staa3]]
[[Category: Dubin G]]
[[Category: Dubin, G]]
[[Category: Golik P]]
[[Category: Golik, P]]
[[Category: Karim A]]
[[Category: Karim, A]]
[[Category: Stach N]]
[[Category: Stach, N]]
[[Category: Bacterial]]
[[Category: Hydrolase]]
[[Category: Protease]]
[[Category: Virulence factor]]

Latest revision as of 08:18, 4 March 2026

Atomic resolution structure of SplF protease from Staphylococcus aureus

6sf7, resolution 1.70Å

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