7zdp

Complex I from Ovis aries at pH9, Open state

spinqualitylabelsall models 7zdp, resolution 3.88Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7zdp is a 10 chain structure with sequence from Ovis aries. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.88Å Ligands: 2MR, AMP, AYA, FES, FME, FMN, K, MYR, NAI, NDP, SEP, ZMP, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function W5PE07_SHEEP Publication Abstract from PubMed Complex I is the first enzyme in the respiratory chain, which is responsible for energy production in mitochondria and bacteria(1). Complex I couples the transfer of two electrons from NADH to quinone and the translocation of four protons across the membrane(2), but the coupling mechanism remains contentious. Here we present cryo-electron microscopy structures of Escherichia coli complex I (EcCI) in different redox states, including catalytic turnover. EcCI exists mostly in the open state, in which the quinone cavity is exposed to the cytosol, allowing access for water molecules, which enable quinone movements. Unlike the mammalian paralogues(3), EcCI can convert to the closed state only during turnover, showing that closed and open states are genuine turnover intermediates. The open-to-closed transition results in the tightly engulfed quinone cavity being connected to the central axis of the membrane arm, a source of substrate protons. Consistently, the proportion of the closed state increases with increasing pH. We propose a detailed but straightforward and robust mechanism comprising a 'domino effect' series of proton transfers and electrostatic interactions: the forward wave ('dominoes stacking') primes the pump, and the reverse wave ('dominoes falling') results in the ejection of all pumped protons from the distal subunit NuoL. This mechanism explains why protons exit exclusively from the NuoL subunit and is supported by our mutagenesis data. We contend that this is a universal coupling mechanism of complex I and related enzymes. A universal coupling mechanism of respiratory complex I.,Kravchuk V, Petrova O, Kampjut D, Wojciechowska-Bason A, Breese Z, Sazanov L Nature. 2022 Sep;609(7928):808-814. doi: 10.1038/s41586-022-05199-7. Epub 2022 , Sep 14. PMID:36104567 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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