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| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8gr7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8gr7 OCA], [https://pdbe.org/8gr7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8gr7 RCSB], [https://www.ebi.ac.uk/pdbsum/8gr7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8gr7 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8gr7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8gr7 OCA], [https://pdbe.org/8gr7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8gr7 RCSB], [https://www.ebi.ac.uk/pdbsum/8gr7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8gr7 ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| | == Function == |
| == Publication Abstract from PubMed == | | [https://www.uniprot.org/uniprot/A0AAJ6N6A9_TAICA A0AAJ6N6A9_TAICA] |
| Antrodia cinnamomea is an endemic species found in Taiwan, known for its medicinal properties in treating various discomforts, including inflammation, diarrhea, abdominal pain, and other diseases. A. cinnamomea contains terpenoids that exhibit numerous bioactivities, making them potential food additives. This discovery piqued our interest in uncovering their biosynthetic pathway. Herein, we conducted functional and structural characterization of a sesquiterpene synthase Cop4 from A. cinnamomea (AcCop4). Through gas chromatography-mass spectrometry analysis, we observed that AcCop4 catalyzes the cyclization of farnesyl pyrophosphate (FPP), primarily producing cubebol. Cubebol is widely used as a long-lasting cooling and refreshing agent in the food industry. The structure of AcCop4, complexed with pyrophosphate and magnesium ions, revealed the closure of the active site facilitated by R311. Interestingly, binding of pyrophosphate and magnesium ions did not cause any significant conformational change in the G1/2 helix of AcCop4, indicating that the apo form is not fully open. This high-resolution structure serves as a solid basis for understanding the biosynthetic mechanism of AcCop4 and supports further production and modification of cubebol for its applications in the food industry.
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| Characterization and Crystal Structures of a Cubebol-Producing Sesquiterpene Synthase from Antrodia cinnamomea.,Chen SC, Jiang BC, Lu YJ, Chang CH, Wu TH, Lin SW, Yin HW, Lee TH, Hsu CH J Agric Food Chem. 2023 Aug 11. doi: 10.1021/acs.jafc.3c00570. PMID:37566786<ref>PMID:37566786</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
| |
| <div class="pdbe-citations 8gr7" style="background-color:#fffaf0;"></div>
| |
| == References ==
| |
| <references/>
| |
| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
