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'''Unreleased structure'''


The entry 9fll is ON HOLD
==Crystal structure of the C-terminal domain of VldE from Streptococcus pneumoniae containing a zinc atom at the binding site==
<StructureSection load='9fll' size='340' side='right'caption='[[9fll]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9fll]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_R6 Streptococcus pneumoniae R6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9FLL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9FLL FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9fll FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9fll OCA], [https://pdbe.org/9fll PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9fll RCSB], [https://www.ebi.ac.uk/pdbsum/9fll PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9fll ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q8DN78_STRR6 Q8DN78_STRR6]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Remodeling of the pneumococcal cell wall, carried out by peptidoglycan (PG) hydrolases, is imperative for maintaining bacterial cell shape and ensuring survival, particularly during cell division or stress response. The Streptococcus pneumoniae protein Spr1875 plays a role in stress response, both regulated by the VicRK two-component system (analogous to the WalRK TCS found in Firmicutes). Modular Spr1875 presents a putative cell-wall binding module at the N-terminus and a catalytic C-terminal module (Spr1875(MT3)) connected by a long linker. Assays of the full-length protein and Spr1875(MT3) with PG-based synthetic substrates by liquid chromatography/mass spectrometry revealed Spr1875 as an l,d-endopeptidase, renamed VldE (for VicRK-regulated l,d-endopeptidase), which hydrolyzed the cross-linked stem peptide in the PG. Remarkably, we observed asymmetric turnover with specific recognition of the acceptor peptide strand. Localization experiments showed that the protein is directed to the septum, which suggests that muralytic activity could be required for pneumococcal growth under stress conditions. Our findings, based on six high-resolution X-ray crystallographic structures and molecular-dynamics simulations, reveal two states for VldE(MT3). The protein transitions between a noncatalytic state that binds up to four zinc ions, thus behaving as a Zn(2+) reservoir, and a catalytic state that performs the hydrolytic reaction with a single zinc ion. Furthermore, computational studies provide insight into the mechanism of catalytic-water activation and nucleophilic attack on the specific scissile peptide bond of the asymmetric cross-linked PG.


Authors: Acebron, I., Miguel-Ruano, V., Hermoso, J.A.
Characterization of VldE (Spr1875), a Pneumococcal Two-State l,d-Endopeptidase with a Four-Zinc Cluster in the Active Site.,Miguel-Ruano V, Acebron I, Lee M, Martin-Galiano AJ, Freton C, de Jose UP, Ramachandran B, Gago F, Kjos M, Hesek D, Grangeasse C, Havarstein LS, Straume D, Mobashery S, Hermoso JA ACS Catal. 2024 Dec 11;14(24):18786-18798. doi: 10.1021/acscatal.4c05090. , eCollection 2024 Dec 20. PMID:39722888<ref>PMID:39722888</ref>


Description: CRYSTAL STRUCTURE OF THE C-TERMINAL DOMAIN OF SPR1875 FROM STREPTOCOCCUS PNEUMONIAE CONTAINING A ZINC ATOM AT THE BINDING SITE
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Miguel-Ruano, V]]
<div class="pdbe-citations 9fll" style="background-color:#fffaf0;"></div>
[[Category: Hermoso, J.A]]
== References ==
[[Category: Acebron, I]]
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptococcus pneumoniae R6]]
[[Category: Acebron I]]
[[Category: Hermoso JA]]
[[Category: Miguel-Ruano V]]
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