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'''Unreleased structure'''


The entry 9twa is ON HOLD
==Legionella monocytogenes SodA mutant GL to VI==
<StructureSection load='9twa' size='340' side='right'caption='[[9twa]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9twa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9TWA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9TWA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9twa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9twa OCA], [https://pdbe.org/9twa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9twa RCSB], [https://www.ebi.ac.uk/pdbsum/9twa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9twa ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Changes in protein properties and functions are central to the evolution of life. Metalloproteins can evolve by changing their preference from one metal cofactor to another. Recently, we demonstrated that the widely distributed iron or manganese dependent superoxide dismutase (SodFM) family have undergone numerous metal-preference changes, including during evolutionary adaptation of pathogenic bacteria to altered metal availability within the host. Yet the underlying properties of metal-binding sites that control metalloenzyme metal-preference are unclear, and thus we lack an understanding of how enzymatic metal-preference can be re-shaped by evolution. Here, we used spectral features of bound iron or manganese, whose intensities reflect their oxidation state, to assess how their redox properties are tuned during SodFM evolution. We systematically analysed the metal oxidation state across diverse SodFMs from multiple phylogenetic groups with different catalytic metal-preferences, including those known to have undergone evolutionary metal-preference switching. We observed a striking relationship between resting oxidation state and catalytic metal-preferences. Mutagenesis of second-sphere residues previously identified as determining metal preference revealed that they modulate metal-dependent activity and cofactor oxidation state in tandem, demonstrating these properties are linked. Together, these data argue that the differing SodFM metal preferences observed across the tree of life evolved through tuning of their redox properties by the secondary coordination sphere. This study gives insight into the process by which a metalloenzyme originally optimised for one metal cofactor can evolve a new metal preference, under suitable selection pressure, through re-optimisation of its active site for catalytic reactivity of the new metal cofactor.


Authors: Mackenzie, E.S., Basle, A., Wldron, K.J.
An enzyme's metal preference evolves through redox modulation driven by the cofactor's secondary coordination sphere.,Mackenzie ES, Sendra KM, Basle A, Mazgaj R, Kehl-Fie TE, Waldron KJ Mol Biol Evol. 2026 Feb 13:msag040. doi: 10.1093/molbev/msag040. PMID:41684149<ref>PMID:41684149</ref>


Description: Legionella monocytogenes SodA mutant GL to VI
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Wldron, K.J]]
<div class="pdbe-citations 9twa" style="background-color:#fffaf0;"></div>
[[Category: Basle, A]]
== References ==
[[Category: Mackenzie, E.S]]
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Listeria monocytogenes]]
[[Category: Basle A]]
[[Category: Mackenzie ES]]
[[Category: Wldron KJ]]
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