1xmt: Difference between revisions
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'''X-ray structure of gene product from arabidopsis thaliana at1g77540''' | '''X-ray structure of gene product from arabidopsis thaliana at1g77540''' | ||
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[[Category: Smith, D W.]] | [[Category: Smith, D W.]] | ||
[[Category: Wesenberg, G E.]] | [[Category: Wesenberg, G E.]] | ||
[[Category: | [[Category: At1g77540]] | ||
[[Category: | [[Category: Center for eukaryotic structural genomic]] | ||
[[Category: | [[Category: Cesg]] | ||
[[Category: | [[Category: Protein structure initiative]] | ||
[[Category: | [[Category: Psi]] | ||
[[Category: | [[Category: Putative acetyltransferase]] | ||
[[Category: | [[Category: Structural genomic]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:14:04 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 12:14, 3 May 2008
X-ray structure of gene product from arabidopsis thaliana at1g77540
Overview
We describe X-ray crystal and NMR solution structures of the protein coded for by Arabidopsis thaliana gene At1g77540.1 (At1g77540). The crystal structure was determined to 1.15 A with an R factor of 14.9% (Rfree = 17.0%) by multiple-wavelength anomalous diffraction using sodium bromide derivatized crystals. The ensemble of NMR conformers was determined with protein samples labeled with 15N and 13C + 15N. The X-ray structure and NMR ensemble were closely similar with rmsd 1.4 A for residues 8-93. At1g77540 was found to adopt a fold similar to that of GCN5-related N-acetyltransferases. Enzymatic activity assays established that At1g77540 possesses weak acetyltransferase activity against histones H3 and H4. Chemical shift perturbations observed in 15N-HSQC spectra upon the addition of CoA indicated that the cofactor binds and identified its binding site. The molecular details of this interaction were further elucidated by solving the X-ray structure of the At1g77540-CoA complex. This work establishes that the domain family COG2388 represents a novel class of acetyltransferase and provides insight into possible mechanistic roles of the conserved Cys76 and His41 residues of this family.
About this Structure
1XMT is a Single protein structure of sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA.
Reference
Structure of Arabidopsis thaliana At1g77540 protein, a minimal acetyltransferase from the COG2388 family., Tyler RC, Bitto E, Berndsen CE, Bingman CA, Singh S, Lee MS, Wesenberg GE, Denu JM, Phillips GN Jr, Markley JL, Biochemistry. 2006 Dec 5;45(48):14325-36. PMID:17128971 Page seeded by OCA on Sat May 3 15:14:04 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Arabidopsis thaliana
- Single protein
- Allard, S T.M.
- Bingman, C A.
- Bitto, E.
- CESG, Center for Eukaryotic Structural Genomics.
- Jr., G N.Phillips.
- Smith, D W.
- Wesenberg, G E.
- At1g77540
- Center for eukaryotic structural genomic
- Cesg
- Protein structure initiative
- Psi
- Putative acetyltransferase
- Structural genomic
