9p6s: Difference between revisions

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'''Unreleased structure'''


The entry 9p6s is ON HOLD  until Paper Publication
==Cryo-EM structure of human integrin alpha5beta1 in complex with fibronectin (FN 7-10)==
<StructureSection load='9p6s' size='340' side='right'caption='[[9p6s]], [[Resolution|resolution]] 2.61&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9p6s]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9P6S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9P6S FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.61&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9p6s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9p6s OCA], [https://pdbe.org/9p6s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9p6s RCSB], [https://www.ebi.ac.uk/pdbsum/9p6s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9p6s ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ITA5_HUMAN ITA5_HUMAN] Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The monoclonal antibody (mAb) TS2/16 stabilizes the active conformation of beta1 integrin, enhancing its adhesive capacity on the cell surface. However, the molecular mechanism by which TS2/16 modulates integrin affinity for extracellular ligands remains unclear. Using endogenous full-length alpha5beta1 integrin purified from human placenta, we determined the structure of integrin alpha5beta1 with fibronectin up to 2.61-A resolution in the absence of TS2/16, capturing the active form without its aid, and performed comparative B-factor-based analysis and CABS-Flex simulation with and without TS2/16. Despite no global conformational differences, we found that TS2/16 interacts with alpha2 helix of the integrin beta1 subunit and contacts the C-terminus of alpha3 helix, leading to a localized decrease in B factor. This interaction allosterically alters the dynamics of alpha2-alpha3 loop despite not being in direct contact with TS2/16. Notably, this loop directly engages fibronectin, and its dynamic change underlies the enhanced ligand-binding affinity and explains increased cell adhesion observed with TS2/16. These findings reveal an allosteric mechanism of integrin regulation by TS2/16 and offer insights for the rational design of therapeutic antibodies targeting integrin-mediated adhesion in pathological contexts such as inflammation and cancer.


Authors:  
Allosteric regulation of fibronectin binding by the anti-beta1 integrin antibody TS2/16.,Ding J, Fantini DA, Dedden D, Schumacher S, Biertumpfel C, Mizuno N PNAS Nexus. 2026 Feb 24;5(3):pgag044. doi: 10.1093/pnasnexus/pgag044. eCollection , 2026 Mar. PMID:41809771<ref>PMID:41809771</ref>


Description:  
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
<div class="pdbe-citations 9p6s" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Biertumpfel C]]
[[Category: Dedden D]]
[[Category: Ding J]]
[[Category: Fantini D]]
[[Category: Mizuno N]]
[[Category: Schumacher S]]
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