9m7w: Difference between revisions
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==At 2S1+S3-tRNA trimer== | |||
<StructureSection load='9m7w' size='340' side='right'caption='[[9m7w]], [[Resolution|resolution]] 3.03Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9m7w]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9M7W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9M7W FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.03Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9m7w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9m7w OCA], [https://pdbe.org/9m7w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9m7w RCSB], [https://www.ebi.ac.uk/pdbsum/9m7w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9m7w ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
S-type anion channel homologs (SLAH) are widely expressed in various plant tissues and play a key role in anion transport, which is crucial for plant adaptation to both biotic and abiotic stresses. In this study, we employ cryo-electron microscopy (cryo-EM) to analyze four SLAH channel complexes from Arabidopsis thaliana: the homotrimeric SLAH3 channel, the 2SLAH1 + SLAH3+tRNA complex, the 1SLAH1 + 2SLAH3 complex, and the 3SLAH1+tRNA complex. Critically, our studies reveal that tRNA directly binds to and occupies the intracellular entrance of the SLAH1 homotrimer and the 2SLAH1 + SLAH3 heterocomplex. Electrophysiological experiments confirm tRNA's role as a potent inhibitory regulatory subunit: RNase-mediated tRNA degradation robustly activates SLAH1 currents, while targeted mutagenesis of SLAH1 tRNA-interacting residues phenocopy this activation and enhanced ABA-induced stomatal closure. Combining with structural biology, electrophysiology, and biochemistry, we comprehensively examine the key residues in SLAH1 and SLAH3 that are responsible for the anion permeation. This mechanistic advancement provides a deeper understanding of the molecular basis for plant stress tolerance and identifies specific molecular targets for future engineering crops. | |||
Structural insights into the coordinated regulation of the SLAH family in Arabidopsis thaliana.,Zhang S, Huang X, Wang X, Qi B, Yang K, Liu C, Li R, Chen X, Yi J, Yin J, Chen M, Liu B, Fan J, Liu T, Hao Z, Cui K, Xiao N, Song Y, Li Y, Wu D, Qi T, Mao J, Zhang B, Yang M, Xie J, Liu Z Nat Commun. 2025 Dec 17;17(1):585. doi: 10.1038/s41467-025-67283-6. PMID:41407702<ref>PMID:41407702</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Zhang | <div class="pdbe-citations 9m7w" style="background-color:#fffaf0;"></div> | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Arabidopsis thaliana]] | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Zhang SS]] | |||
