9ywn: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==Protein Structure of the First Glycoside Hydrolase Family 30, Subfamily 12 Endoxylanase== | |||
<StructureSection load='9ywn' size='340' side='right'caption='[[9ywn]], [[Resolution|resolution]] 1.92Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9ywn]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Oscillospiraceae Oscillospiraceae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9YWN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9YWN FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.916Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9ywn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9ywn OCA], [https://pdbe.org/9ywn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9ywn RCSB], [https://www.ebi.ac.uk/pdbsum/9ywn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9ywn ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
We have determined the X-ray crystallographic protein structure of endo-1,4-beta-xylanase (EX) A from Anaerobacterium chartisolvens (AchXyn30A), a homologue of the recent biochemically characterized glycoside hydrolase family 30, subfamily 12 (GH30_12) EX from Acetivibrio clariflavus (AcXyn30B). The N-terminal GH30 catalytic domains (CDs) of these two enzymes share approximately 63% amino-acid sequence identity and the full-length proteins each consist of the GH30_12 CD, a family 6 carbohydrate-binding module and a C-terminal dockerin domain. In this report, we offer additional support for the recent subfamily classification of these EXs and provide detailed X-ray crystallographic protein structure analysis of AchXyn30A, the first protein structure from this newly defined GH30 subfamily. We also provide comparative structural analysis using a generated AcXyn30B homology model as well as other GH30 subfamily enzymes. Additionally, we examine potential xylan-chain interactions informed by the protein structure. These characterized EXs further illustrate the diversity of xylan-degrading enzymes which have evolved within glycoside hydrolase family 30. | |||
Protein structure of a glycoside hydrolase family 30, subfamily 12 endo-1,4-beta-xylanase.,St John FJ, Crooks C, Endres M, Pakdaman L, Koch L, Bynum L, Kuch N, Joachimiak A, Tan K Acta Crystallogr D Struct Biol. 2026 Apr 1;82(Pt 4):370-382. doi: , 10.1107/S2059798326002160. Epub 2026 Mar 23. PMID:41870978<ref>PMID:41870978</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 9ywn" style="background-color:#fffaf0;"></div> | ||
[[Category: St John | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Oscillospiraceae]] | |||
[[Category: St John FJ]] | |||
[[Category: Tan K]] | |||
