9o5h: Difference between revisions
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==Crystal structure of GH30_8 xylanase from Bacillus pumilus== | |||
<StructureSection load='9o5h' size='340' side='right'caption='[[9o5h]], [[Resolution|resolution]] 2.16Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9o5h]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_pumilus_ATCC_7061 Bacillus pumilus ATCC 7061]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9O5H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9O5H FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.16Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9o5h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9o5h OCA], [https://pdbe.org/9o5h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9o5h RCSB], [https://www.ebi.ac.uk/pdbsum/9o5h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9o5h ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Transformation of agro-industrial products into value-added products, such as prebiotic oligosaccharides, is a key element of the emerging bioeconomy. Here, we characterized a new GH30_8 glucuronoxylanase from Bacillus pumilus (BpXyn30_8A) for its potential in producing xylooligosaccharides (XOS). BpXyn30_8A showed tolerance to ethanol and NaCl and released both linear and branched XOS containing MeGlcA at the penultimate nonreducing end residue. Its X-ray structure, determined at 2.16 A resolution, revealed high similarity to other glucuronoxylanases. Furthermore, BpXyn30_8A achieved higher xylan conversion yields from corn cob and Eucalyptus sawdust than Ruminococcus champanellensisRcXyn30A. Finally, fermentation assays showed that Bifidobacterium adolescentis metabolized neutral XOS to acetate and lactate, whereas acidic XOS were poorly utilized. These results highlight the potential of BpXyn30_8A as a valuable enzyme for the green transformation of plant biomass into prebiotic oligosaccharides with promising applications in human and animal nutrition, health, and biotechnology. | |||
Enzymatic Production of Prebiotic Xylooligosaccharides Using a Bacillus pumilus GH30_8 Glucuronoxylanase: Structural Basis of Glucuronoxylan Recognition and Hydrolysis.,Vacilotto MM, Pellegrini VOA, Araujo EA, Liberato MV, Polikarpov I J Agric Food Chem. 2026 Feb 18;74(6):5417-5430. doi: 10.1021/acs.jafc.5c07569. , Epub 2026 Feb 3. PMID:41632704<ref>PMID:41632704</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 9o5h" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Bacillus pumilus ATCC 7061]] | |||
[[Category: Large Structures]] | |||
[[Category: Araujo EA]] | |||
[[Category: Polikarpov I]] | |||
[[Category: Vacilotto MM]] | |||
Latest revision as of 08:11, 29 April 2026
Crystal structure of GH30_8 xylanase from Bacillus pumilus
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