11bd: Difference between revisions

Latest revision as of 03:35, 14 May 2026

human nucleosome assembly protein 1 (human Nap1)

Structural highlights

11bd is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.2Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NP1L1_HUMAN Histone chaperone that plays a role in the nuclear import of H2A-H2B and nucleosome assembly (PubMed:20002496, PubMed:21211722, PubMed:26841755). Also participates in several important DNA repair mechanisms: greatly enhances ERCC6-mediated chromatin remodeling which is essential for transcription-coupled nucleotide excision DNA repair (PubMed:28369616). Also stimulates homologous recombination (HR) by RAD51 and RAD54 which is essential in mitotic DNA double strand break (DSB) repair (PubMed:24798879). Plays a key role in the regulation of embryonic neurogenesis (By similarity). Promotes the proliferation of neural progenitors and inhibits neuronal differentiation during cortical development (By similarity). Regulates neurogenesis via the modulation of RASSF10; regulates RASSF10 expression by promoting SETD1A-mediated H3K4 methylation at the RASSF10 promoter (By similarity).[UniProtKB:P28656]^[1] ^[2] ^[3] ^[4] ^[5] (Microbial infection) Positively regulates Epstein-Barr virus reactivation in epithelial cells through the induction of viral BZLF1 expression.^[6] (Microbial infection) Together with human herpesvirus 8 protein LANA1, assists the proper assembly of the nucleosome on the replicated viral DNA.^[7]

References

↑ Okuwaki M, Kato K, Nagata K. Functional characterization of human nucleosome assembly protein 1-like proteins as histone chaperones. Genes Cells. 2010 Jan;15(1):13-27. PMID:20002496 doi:10.1111/j.1365-2443.2009.01361.x
↑ Mehrotra PV, Ahel D, Ryan DP, Weston R, Wiechens N, Kraehenbuehl R, Owen-Hughes T, Ahel I. DNA repair factor APLF is a histone chaperone. Mol Cell. 2011 Jan 7;41(1):46-55. PMID:21211722 doi:10.1016/j.molcel.2010.12.008
↑ Machida S, Takaku M, Ikura M, Sun J, Suzuki H, Kobayashi W, Kinomura A, Osakabe A, Tachiwana H, Horikoshi Y, Fukuto A, Matsuda R, Ura K, Tashiro S, Ikura T, Kurumizaka H. Nap1 stimulates homologous recombination by RAD51 and RAD54 in higher-ordered chromatin containing histone H1. Sci Rep. 2014 May 6;4:4863. PMID:24798879 doi:10.1038/srep04863
↑ Ohtomo H, Akashi S, Moriwaki Y, Okuwaki M, Osakabe A, Nagata K, Kurumizaka H, Nishimura Y. C-terminal acidic domain of histone chaperone human NAP1 is an efficient binding assistant for histone H2A-H2B, but not H3-H4. Genes Cells. 2016 Mar;21(3):252-63. PMID:26841755 doi:10.1111/gtc.12339
↑ Lee JY, Lake RJ, Kirk J, Bohr VA, Fan HY, Hohng S. NAP1L1 accelerates activation and decreases pausing to enhance nucleosome remodeling by CSB. Nucleic Acids Res. 2017 May 5;45(8):4696-4707. PMID:28369616 doi:10.1093/nar/gkx188
↑ Mansouri S, Wang S, Frappier L. A role for the nucleosome assembly proteins TAF-Iβ and NAP1 in the activation of BZLF1 expression and Epstein-Barr virus reactivation. PLoS One. 2013 May 14;8(5):e63802. PMID:23691099 doi:10.1371/journal.pone.0063802
↑ Gupta N, Thakker S, Verma SC. KSHV encoded LANA recruits Nucleosome Assembly Protein NAP1L1 for regulating viral DNA replication and transcription. Sci Rep. 2016 Sep 7;6:32633. PMID:27599637 doi:10.1038/srep32633

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Okuwaki M, Kato K, Nagata K. Functional characterization of human nucleosome assembly protein 1-like proteins as histone chaperones. Genes Cells. 2010 Jan;15(1):13-27. PMID:20002496 doi:10.1111/j.1365-2443.2009.01361.x

[2] Mehrotra PV, Ahel D, Ryan DP, Weston R, Wiechens N, Kraehenbuehl R, Owen-Hughes T, Ahel I. DNA repair factor APLF is a histone chaperone. Mol Cell. 2011 Jan 7;41(1):46-55. PMID:21211722 doi:10.1016/j.molcel.2010.12.008

[3] Machida S, Takaku M, Ikura M, Sun J, Suzuki H, Kobayashi W, Kinomura A, Osakabe A, Tachiwana H, Horikoshi Y, Fukuto A, Matsuda R, Ura K, Tashiro S, Ikura T, Kurumizaka H. Nap1 stimulates homologous recombination by RAD51 and RAD54 in higher-ordered chromatin containing histone H1. Sci Rep. 2014 May 6;4:4863. PMID:24798879 doi:10.1038/srep04863

[4] Ohtomo H, Akashi S, Moriwaki Y, Okuwaki M, Osakabe A, Nagata K, Kurumizaka H, Nishimura Y. C-terminal acidic domain of histone chaperone human NAP1 is an efficient binding assistant for histone H2A-H2B, but not H3-H4. Genes Cells. 2016 Mar;21(3):252-63. PMID:26841755 doi:10.1111/gtc.12339

[5] Lee JY, Lake RJ, Kirk J, Bohr VA, Fan HY, Hohng S. NAP1L1 accelerates activation and decreases pausing to enhance nucleosome remodeling by CSB. Nucleic Acids Res. 2017 May 5;45(8):4696-4707. PMID:28369616 doi:10.1093/nar/gkx188

[6] Mansouri S, Wang S, Frappier L. A role for the nucleosome assembly proteins TAF-Iβ and NAP1 in the activation of BZLF1 expression and Epstein-Barr virus reactivation. PLoS One. 2013 May 14;8(5):e63802. PMID:23691099 doi:10.1371/journal.pone.0063802

[7] Gupta N, Thakker S, Verma SC. KSHV encoded LANA recruits Nucleosome Assembly Protein NAP1L1 for regulating viral DNA replication and transcription. Sci Rep. 2016 Sep 7;6:32633. PMID:27599637 doi:10.1038/srep32633

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 11bd is ON HOLD  until Paper Publication
+==human nucleosome assembly protein 1 (human Nap1)==
+<StructureSection load='11bd' size='340' side='right'caption='[[11bd]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
-Authors: Eren, E.
+== Structural highlights ==
+<table><tr><td colspan='2'>[[11bd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=11BD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=11BD FirstGlance]. <br>
-Description: human nucleosome assembly protein 1 (human Nap1)
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
-[[Category: Unreleased Structures]]
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=11bd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=11bd OCA], [https://pdbe.org/11bd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=11bd RCSB], [https://www.ebi.ac.uk/pdbsum/11bd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=11bd ProSAT]</span></td></tr>
-[[Category: Eren, E]]
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/NP1L1_HUMAN NP1L1_HUMAN] Histone chaperone that plays a role in the nuclear import of H2A-H2B and nucleosome assembly (PubMed:20002496, PubMed:21211722, PubMed:26841755). Also participates in several important DNA repair mechanisms: greatly enhances ERCC6-mediated chromatin remodeling which is essential for transcription-coupled nucleotide excision DNA repair (PubMed:28369616). Also stimulates homologous recombination (HR) by RAD51 and RAD54 which is essential in mitotic DNA double strand break (DSB) repair (PubMed:24798879). Plays a key role in the regulation of embryonic neurogenesis (By similarity). Promotes the proliferation of neural progenitors and inhibits neuronal differentiation during cortical development (By similarity). Regulates neurogenesis via the modulation of RASSF10; regulates RASSF10 expression by promoting SETD1A-mediated H3K4 methylation at the RASSF10 promoter (By similarity).[UniProtKB:P28656]<ref>PMID:20002496</ref> <ref>PMID:21211722</ref> <ref>PMID:24798879</ref> <ref>PMID:26841755</ref> <ref>PMID:28369616</ref>   (Microbial infection) Positively regulates Epstein-Barr virus reactivation in epithelial cells through the induction of viral BZLF1 expression.<ref>PMID:23691099</ref>   (Microbial infection) Together with human herpesvirus 8 protein LANA1, assists the proper assembly of the nucleosome on the replicated viral DNA.<ref>PMID:27599637</ref>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Homo sapiens]]
+[[Category: Large Structures]]
+[[Category: Eren E]]

11bd: Difference between revisions

Latest revision as of 03:35, 14 May 2026

human nucleosome assembly protein 1 (human Nap1)

Structural highlights

Function

References

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