9o1m: Difference between revisions

Latest revision as of 04:06, 14 May 2026

TMEM16F in liposomes in the presence of Ca2+ (closed state)

Structural highlights

9o1m is a 2 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 2.92Å
Ligands:	CA, PGW
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ANO6_MOUSE Small-conductance calcium-activated nonselective cation (SCAN) channel which acts as a regulator of phospholipid scrambling in platelets, osteoblasts and fetal thymocytes. Phospholipid scrambling results in surface exposure of phosphatidylserine which in platelets is essential to trigger the clotting system whereas in osteoblasts is essential for the deposition of hydroxyapatite during bone mineralization. Has calcium-dependent phospholipid scramblase activity; scrambles phosphatidylserine, phosphatidylcholine and galactosylceramide. Can generate outwardly rectifying chloride channel currents in airway epithelial cells and Jurkat T lymphocytes.^[1] ^[2] ^[3] ^[4] ^[5]

References

↑ Suzuki J, Umeda M, Sims PJ, Nagata S. Calcium-dependent phospholipid scrambling by TMEM16F. Nature. 2010 Dec 9;468(7325):834-8. doi: 10.1038/nature09583. Epub 2010 Nov 24. PMID:21107324 doi:https://dx.doi.org/10.1038/nature09583
↑ Kunzelmann K, Schreiber R, Kmit A, Jantarajit W, Martins JR, Faria D, Kongsuphol P, Ousingsawat J, Tian Y. Expression and function of epithelial anoctamins. Exp Physiol. 2012 Feb;97(2):184-92. doi: 10.1113/expphysiol.2011.058206. Epub, 2011 Sep 9. PMID:21908539 doi:https://dx.doi.org/10.1113/expphysiol.2011.058206
↑ Ehlen HW, Chinenkova M, Moser M, Munter HM, Krause Y, Gross S, Brachvogel B, Wuelling M, Kornak U, Vortkamp A. Inactivation of anoctamin-6/Tmem16f, a regulator of phosphatidylserine scrambling in osteoblasts, leads to decreased mineral deposition in skeletal tissues. J Bone Miner Res. 2013 Feb;28(2):246-59. doi: 10.1002/jbmr.1751. PMID:22936354 doi:https://dx.doi.org/10.1002/jbmr.1751
↑ Yang H, Kim A, David T, Palmer D, Jin T, Tien J, Huang F, Cheng T, Coughlin SR, Jan YN, Jan LY. TMEM16F forms a Ca2+-activated cation channel required for lipid scrambling in platelets during blood coagulation. Cell. 2012 Sep 28;151(1):111-22. doi: 10.1016/j.cell.2012.07.036. PMID:23021219 doi:https://dx.doi.org/10.1016/j.cell.2012.07.036
↑ Suzuki J, Fujii T, Imao T, Ishihara K, Kuba H, Nagata S. Calcium-dependent phospholipid scramblase activity of TMEM16 protein family members. J Biol Chem. 2013 May 10;288(19):13305-16. doi: 10.1074/jbc.M113.457937. Epub, 2013 Mar 26. PMID:23532839 doi:https://dx.doi.org/10.1074/jbc.M113.457937

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OCA

[1] Suzuki J, Umeda M, Sims PJ, Nagata S. Calcium-dependent phospholipid scrambling by TMEM16F. Nature. 2010 Dec 9;468(7325):834-8. doi: 10.1038/nature09583. Epub 2010 Nov 24. PMID:21107324 doi:https://dx.doi.org/10.1038/nature09583

[2] Kunzelmann K, Schreiber R, Kmit A, Jantarajit W, Martins JR, Faria D, Kongsuphol P, Ousingsawat J, Tian Y. Expression and function of epithelial anoctamins. Exp Physiol. 2012 Feb;97(2):184-92. doi: 10.1113/expphysiol.2011.058206. Epub, 2011 Sep 9. PMID:21908539 doi:https://dx.doi.org/10.1113/expphysiol.2011.058206

[3] Ehlen HW, Chinenkova M, Moser M, Munter HM, Krause Y, Gross S, Brachvogel B, Wuelling M, Kornak U, Vortkamp A. Inactivation of anoctamin-6/Tmem16f, a regulator of phosphatidylserine scrambling in osteoblasts, leads to decreased mineral deposition in skeletal tissues. J Bone Miner Res. 2013 Feb;28(2):246-59. doi: 10.1002/jbmr.1751. PMID:22936354 doi:https://dx.doi.org/10.1002/jbmr.1751

[4] Yang H, Kim A, David T, Palmer D, Jin T, Tien J, Huang F, Cheng T, Coughlin SR, Jan YN, Jan LY. TMEM16F forms a Ca2+-activated cation channel required for lipid scrambling in platelets during blood coagulation. Cell. 2012 Sep 28;151(1):111-22. doi: 10.1016/j.cell.2012.07.036. PMID:23021219 doi:https://dx.doi.org/10.1016/j.cell.2012.07.036

[5] Suzuki J, Fujii T, Imao T, Ishihara K, Kuba H, Nagata S. Calcium-dependent phospholipid scramblase activity of TMEM16 protein family members. J Biol Chem. 2013 May 10;288(19):13305-16. doi: 10.1074/jbc.M113.457937. Epub, 2013 Mar 26. PMID:23532839 doi:https://dx.doi.org/10.1074/jbc.M113.457937

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 9o1m is ON HOLD  until Paper Publication
+==TMEM16F in liposomes in the presence of Ca2+ (closed state)==
+<StructureSection load='9o1m' size='340' side='right'caption='[[9o1m]], [[Resolution|resolution]] 2.92&Aring;' scene=''>
-Authors: Feng, Z., Accardi, A.
+== Structural highlights ==
+<table><tr><td colspan='2'>[[9o1m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9O1M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9O1M FirstGlance]. <br>
-Description: TMEM16F in liposomes in the presence of Ca2+ (closed state)
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.92&#8491;</td></tr>
-[[Category: Unreleased Structures]]
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PGW:(1R)-2-{[(S)-{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(HEXADECANOYLOXY)METHYL]ETHYL+(9Z)-OCTADEC-9-ENOATE'>PGW</scene></td></tr>
-[[Category: Feng, Z]]
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9o1m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9o1m OCA], [https://pdbe.org/9o1m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9o1m RCSB], [https://www.ebi.ac.uk/pdbsum/9o1m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9o1m ProSAT]</span></td></tr>
-[[Category: Accardi, A]]
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/ANO6_MOUSE ANO6_MOUSE] Small-conductance calcium-activated nonselective cation (SCAN) channel which acts as a regulator of phospholipid scrambling in platelets, osteoblasts and fetal thymocytes. Phospholipid scrambling results in surface exposure of phosphatidylserine which in platelets is essential to trigger the clotting system whereas in osteoblasts is essential for the deposition of hydroxyapatite during bone mineralization. Has calcium-dependent phospholipid scramblase activity; scrambles phosphatidylserine, phosphatidylcholine and galactosylceramide. Can generate outwardly rectifying chloride channel currents in airway epithelial cells and Jurkat T lymphocytes.<ref>PMID:21107324</ref> <ref>PMID:21908539</ref> <ref>PMID:22936354</ref> <ref>PMID:23021219</ref> <ref>PMID:23532839</ref>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Mus musculus]]
+[[Category: Accardi A]]
+[[Category: Feng Z]]

9o1m: Difference between revisions

Latest revision as of 04:06, 14 May 2026

TMEM16F in liposomes in the presence of Ca2+ (closed state)

Structural highlights

Function

References

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