1y0v: Difference between revisions
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'''Crystal structure of anthrax edema factor (EF) in complex with calmodulin and pyrophosphate''' | '''Crystal structure of anthrax edema factor (EF) in complex with calmodulin and pyrophosphate''' | ||
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[[Category: Tang, W J.]] | [[Category: Tang, W J.]] | ||
[[Category: Zhukovskaya, N L.]] | [[Category: Zhukovskaya, N L.]] | ||
[[Category: | [[Category: Anthrax edema factor]] | ||
[[Category: | [[Category: Calcium-independent]] | ||
[[Category: | [[Category: Calmodulin]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:44:55 2008'' | |||
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Revision as of 12:44, 3 May 2008
Crystal structure of anthrax edema factor (EF) in complex with calmodulin and pyrophosphate
Overview
Edema factor (EF), a key anthrax exotoxin, has an anthrax protective antigen-binding domain (PABD) and a calmodulin (CaM)-activated adenylyl cyclase domain. Here, we report the crystal structures of CaM-bound EF, revealing the architecture of EF PABD. CaM has N- and C-terminal domains and each domain can bind two calcium ions. Calcium binding induces the conformational change of CaM from closed to open. Structures of the EF-CaM complex show how EF locks the N-terminal domain of CaM into a closed conformation regardless of its calcium-loading state. This represents a mechanism of how CaM effector alters the calcium affinity of CaM and uncouples the conformational change of CaM from calcium loading. Furthermore, structures of EF-CaM complexed with nucleotides show that EF uses two-metal-ion catalysis, a prevalent mechanism in DNA and RNA polymerases. A histidine (H351) further facilitates the catalysis of EF by activating a water to deprotonate 3'OH of ATP. Mammalian adenylyl cyclases share no structural similarity with EF and they also use two-metal-ion catalysis, suggesting the catalytic mechanism-driven convergent evolution of two structurally diverse adenylyl cyclases.
About this Structure
1Y0V is a Protein complex structure of sequences from Bacillus anthracis and Xenopus laevis. Full crystallographic information is available from OCA.
Reference
Calcium-independent calmodulin binding and two-metal-ion catalytic mechanism of anthrax edema factor., Shen Y, Zhukovskaya NL, Guo Q, Florian J, Tang WJ, EMBO J. 2005 Mar 9;24(5):929-41. Epub 2005 Feb 17. PMID:15719022 Page seeded by OCA on Sat May 3 15:44:55 2008
