9x0k: Difference between revisions

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'''Unreleased structure'''


The entry 9x0k is ON HOLD  until Paper Publication
==Cryo-EM Structure of Turbo sazae ferritin chain A==
<StructureSection load='9x0k' size='340' side='right'caption='[[9x0k]], [[Resolution|resolution]] 2.19&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9x0k]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Turbo_sazae Turbo sazae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9X0K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9X0K FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.19&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9x0k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9x0k OCA], [https://pdbe.org/9x0k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9x0k RCSB], [https://www.ebi.ac.uk/pdbsum/9x0k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9x0k ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Turbo sazae, an edible gastropod, accumulates high levels of iron in its digestive gland, and the molecular mechanism underlying this accumulation has remained elusive. This study identified the proteins responsible for the iron accumulation and characterized their function and structure. We isolated two novel ferritins, TsFerA and TsFerB, from the digestive gland using high-performance liquid chromatography and inductively coupled plasma mass spectrometry. Gene expression analyses revealed that both genes were specifically expressed in the digestive gland. Recombinant TsFerA (rTsFerA) possessed ferroxidase (EC1.16.3.1) activity, whereas recombinant TsFerB (rTsFerB) showed no such activity. This result indicated that rTsFerA functions as an H-chain-like subunit responsible for iron oxidation, while rTsFerB acts as an L-chain-like subunit involved in iron core nucleation. Furthermore, we determined the structures of rTsFerA and rTsFerB using cryo-electron microscopy, at a resolution of 2.19 and 2.17 A, respectively. The protein structures revealed a conserved ferroxidase center in rTsFerA, whereas key catalytic residues were substituted in rTsFerB. These findings demonstrate that T. sazae utilizes a cooperative system composed of two functionally distinct ferritin subunits to efficiently and safely store iron. This work clarifies the molecular mechanisms of iron metabolism in marine organisms, particularly in gastropods, revealing an optimized strategy to cope with massive iron influx.


Authors:  
Identification, functional characterization, and cryo-EM structural analysis of novel ferritin subunits in Turbo sazae.,Namikawa Y, Negishi L, Kurumizaka H, Suzuki M FEBS J. 2026 May 26. doi: 10.1111/febs.70601. PMID:42187216<ref>PMID:42187216</ref>


Description:  
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
<div class="pdbe-citations 9x0k" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Turbo sazae]]
[[Category: Namikawa Y]]
[[Category: Suzuki M]]
Retrieved from "https://proteopedia.org/9x0k"