9z0b: Difference between revisions

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'''Unreleased structure'''


The entry 9z0b is ON HOLD  until Paper Publication
==Crystal Structure of the Polycaprolactam (Nylon6) and Poly(Hexamethylene Adipamide) (Nylon66) Hydrolase Nyl12 at Cryo Temperature==
<StructureSection load='9z0b' size='340' side='right'caption='[[9z0b]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9z0b]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Gammaproteobacteria_bacterium Gammaproteobacteria bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9Z0B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9Z0B FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CHT:CHOLINE+ION'>CHT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9z0b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9z0b OCA], [https://pdbe.org/9z0b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9z0b RCSB], [https://www.ebi.ac.uk/pdbsum/9z0b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9z0b ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Enzymatic degradation of synthetic polymers has attracted broad interest because it offers environmental and manufacturing advantages compared to traditional mechanical and chemical breakdown approaches. Enzymes are highly specific and reaction conditions are generally aqueous and require low pressure and temperature, resulting in lower energy consumption and lower chemical waste production. Here we report the biochemical and structural characterization of three newly discovered enzymes capable of nylon hydrolysis: Nyl10, Nyl12 and Nyl50. Using solution characterization techniques, we found that the enzymes adopt a single oligomeric state consistent with a tetramer over a wide range of concentrations. X-ray crystallographic structures of all three enzymes support the association into tetramers. Comparison of ligand-bound X-ray crystal structures of Nyl10 and Nyl12 with the previously determined structure of Nyl50 identified key structural determinants involved in ligand binding. Noticeably, a flexible loop found in several polyamide degrading enzymes is observed to flip towards (closed conformation) and away (open conformation) from the active site upon ligand binding. Analysis of adduct and surrogate substrate-bound enzyme complex structures provide a model for substrate binding directionality. Finally, activity assays showed that both Nyl10 and Nyl12 can hydrolyze ester bonds, and that Nyl12 has the highest activity toward PA66, identifying it as the best candidate for protein engineering for efficient nylon hydrolysis.


Authors: Capra, N., Meilleur, F.
Structural and oligomeric characterization of substrate- and product-selective nylon hydrolases.,Capra N, Bourgery C, Parks JM, Carper DL, Cahill JF, Michener JK, Meilleur F bioRxiv [Preprint]. 2026 Mar 19:2026.03.11.711162. doi: , 10.64898/2026.03.11.711162. PMID:41889879<ref>PMID:41889879</ref>


Description: Crystal Structure of the Polycaprolactam (Nylon6) and Poly(Hexamethylene Adipamide) (Nylon66) Hydrolase Nyl12 at Cryo Temperature
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Capra, N]]
<div class="pdbe-citations 9z0b" style="background-color:#fffaf0;"></div>
[[Category: Meilleur, F]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Gammaproteobacteria bacterium]]
[[Category: Large Structures]]
[[Category: Capra N]]
[[Category: Meilleur F]]
Retrieved from "https://proteopedia.org/9z0b"