9za2

cryoEM structure of COMMD-like protein S4Y171 octamer

spinqualitylabelsall models 9za2, resolution 4.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9za2 is a 8 chain structure with sequence from Sorangium cellulosum So0157-2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 4.7Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function S4XVP2_SORCE Publication Abstract from PubMed The ten eukaryotic COMMD proteins are core components of the Commander complex, with central roles in endosomal membrane trafficking and signalling. Each protein has an alpha-helical N-terminal (HN) domain with a C-terminal copper metabolism gene MURR1 (COMM) domain. These ten family members assemble into a heterodecameric ring composed of five specific heterodimers. In this work we have combined structural homology searches with genome-wide predicted structures to identify ancestral COMMD-like proteins that exist as single genes in Bacteria and Archaea. Although there is limited sequence similarity to the eukaryotic proteins the bacterial and archaeal COMMD-like proteins are predicted to form homomeric ring-shaped assemblies like their eukaryotic counterparts. Our biophysical studies, crystal and cryo-EM structures confirm COMMD-like proteins readily form homooligomeric rings composed of eight or ten subunits assembled from core dimeric building blocks and inter-dimer interactions that are analogous to the heterodecameric core structure of the eukaryotic Commander complex. Phylogenetic analyses using amino acid sequences and FoldSeek structural alphabet (3Di) infer that the closest identified relatives to the eukaryotic COMMD proteins are found in Myxococcota bacteria. These findings indicate that COMMD genes emerged early in eukaryotic evolution through multiple rounds of duplication from a single ancestral gene likely acquired from bacteria. The prokaryotic origins of the COMMD protein family involved in eukaryotic membrane trafficking.,Liu M, Moody ERR, Blades F, Puente-Lelievre C, Chen KE, Stephens EJ, Michie KA, Cater RJ, Williams TA, Collins BM, Healy MD Nat Commun. 2026 May 29. doi: 10.1038/s41467-026-73536-9. PMID:42215467 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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