9vrf: Difference between revisions
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==Fundamental structural studies of metanaphthene and introduction of disulfide bonds== | |||
<StructureSection load='9vrf' size='340' side='right'caption='[[9vrf]], [[Resolution|resolution]] 1.45Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9vrf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9VRF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9VRF FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A1ES9:naphthalen-1-yl+~{N}-methylcarbamate'>A1ES9</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9vrf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9vrf OCA], [https://pdbe.org/9vrf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9vrf RCSB], [https://www.ebi.ac.uk/pdbsum/9vrf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9vrf ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The unique characteristics of nanobodies make them ideal candidates for diagnostic and therapeutic applications. Introducing additional disulfide bonds is one of the most effective ways to enhance a nanobody's stability. However, it always negatively affects the nanobody's affinity and expression in host cells. In this study, an anticarbaryl nanobody (NbCBR) was used as a model to investigate the strategy of additional disulfide bond introduction. The crystal structure of the NbCBR-carbaryl complex was determined, based on which molecular dynamics simulations and virtual screening were carried out. Seven disulfide bond-introduced mutants were then constructed. Compared with the wild-type nanobody, all the mutants retained good sensitivity. The results demonstrate that introducing disulfide bonds between two beta-sheets or anchoring the flexible CDR3 to a beta-sheet can significantly enhance the nanobody's stability under thermal, organic solvent, and pH stress conditions. This study provides a theoretical basis for rational engineering of nanobodies to improve their stability, potentially expanding their further applications. | |||
An Effective Way to Introduce Disulfide Bonds into Nanobodies: Using an Anticarbaryl Nanobody Structure as a Model.,Liang CX, Liu ML, Guo PY, Fang RY, Hendrickson OD, Zherdev AV, Wang H, Xu ZL, Shen X J Agric Food Chem. 2026 Jul 1;74(25):19995-20004. doi: 10.1021/acs.jafc.6c03602. , Epub 2026 Jun 22. PMID:42328891<ref>PMID:42328891</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Liang | <div class="pdbe-citations 9vrf" style="background-color:#fffaf0;"></div> | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Synthetic construct]] | |||
[[Category: Liang C]] | |||
Latest revision as of 12:58, 1 July 2026
Fundamental structural studies of metanaphthene and introduction of disulfide bonds
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