9wa8: Difference between revisions

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'''Unreleased structure'''


The entry 9wa8 is ON HOLD  until Paper Publication
==Crystal structure of AcrIIA19 from Staphylococcus pseudintermedius==
<StructureSection load='9wa8' size='340' side='right'caption='[[9wa8]], [[Resolution|resolution]] 1.98&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9wa8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_pseudintermedius Staphylococcus pseudintermedius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9WA8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9WA8 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.98&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9wa8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9wa8 OCA], [https://pdbe.org/9wa8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9wa8 RCSB], [https://www.ebi.ac.uk/pdbsum/9wa8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9wa8 ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Anti-CRISPR (Acr) proteins are natural inhibitors of clustered regularly interspaced short palindromic repeat (CRISPR)-CRISPR-associated protein (Cas) systems, providing valuable tools for regulating genome editing. Here, we present the crystal structure of AcrIIA19, a plasmid-encoded Type II-A CRISPR-Cas system inhibitor that targets Cas9. AcrIIA19 adopts a previously uncharacterized fold and forms a stable homodimer. Biochemical assays revealed that AcrIIA19 binds selectively to the wedge (WED) domain of Cas9, a conserved structural interface critical for single guide RNA-DNA duplex stabilization and catalysis. This interaction disrupts Cas9 activity at multiple stages, independent of the order of complex assembly. Notably, AcrIIA19 exhibits broad-spectrum inhibition across divergent Cas9 orthologs, including Streptococcus pyogenes and Staphylococcus aureus Cas9, by exploiting a conserved WED domain vulnerability. Our findings establish AcrIIA19 as a versatile Cas9 inhibitor and highlight the WED domain as a strategic target for developing species-agnostic CRISPR regulatory tools in biotechnology and therapeutic applications.


Authors: Kim, G.E., Kang, Y.J., Park, H.H.
AcrIIA19 binds to the WED domain and inhibits various Cas9 orthologs at multiple stages.,Kim GE, Lee SY, Kang YJ, Bin Jin H, Park HH Commun Biol. 2025 Dec 23;9(1):136. doi: 10.1038/s42003-025-09417-6. PMID:41430372<ref>PMID:41430372</ref>


Description: Crystal structure of AcrIIA19 from Staphylococcus pseudintermedius
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Park, H.H]]
<div class="pdbe-citations 9wa8" style="background-color:#fffaf0;"></div>
[[Category: Kang, Y.J]]
== References ==
[[Category: Kim, G.E]]
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Staphylococcus pseudintermedius]]
[[Category: Kang YJ]]
[[Category: Kim GE]]
[[Category: Park HH]]
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