5fs0

crystal structure of the phenol-responsive sensory domain of the transcription activator PoxR with 2,4-dichlorophenol

spinqualitylabelsall models 5fs0, resolution 2.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5fs0 is a 2 chain structure with sequence from Ralstonia sp. E2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.4Å Ligands: 5JC, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function O84957_9RALS Publication Abstract from PubMed Positive phenol-degradative gene regulator (PoxR) is a sigma(54)-dependent AAA+ ATPase transcription activator that regulates the catabolism of phenols. The PoxR sensory domain detects phenols and relays signals for the activation of transcription. Here we report the first structure of the phenol sensory domain bound to phenol and five derivatives. It exists as a tightly intertwined homodimer with a phenol-binding pocket buried inside, placing two C termini on the same side of the dimer. His102 and Trp130 interact with the hydroxyl group of the phenol in a cavity surrounded by rigid hydrophobic residues on one side and a flexible region on the other. Each monomer has a V4R fold with a unique zinc-binding site. A shift at the C-terminal helix suggests that there is a possible conformational change upon ligand binding. The results provide a structural basis of chemical effector binding for transcriptional regulation with broad implications for protein engineering. Structural Analysis of the Phenol-Responsive Sensory Domain of the Transcription Activator PoxR.,Patil VV, Park KH, Lee SG, Woo E Structure. 2016 Apr 5;24(4):624-30. doi: 10.1016/j.str.2016.03.006. PMID:27050690 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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