23ks: Difference between revisions
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==Crystal structure of AprI/SAH complex== | |||
<StructureSection load='23ks' size='340' side='right'caption='[[23ks]], [[Resolution|resolution]] 2.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[23ks]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptoalloteichus_tenebrarius Streptoalloteichus tenebrarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=23KS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=23KS FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAO:5-S-[(3S)-3-AZANIUMYL-3-CARBOXYPROPYL]-5-THIOADENOSINE'>SAO</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=23ks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=23ks OCA], [https://pdbe.org/23ks PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=23ks RCSB], [https://www.ebi.ac.uk/pdbsum/23ks PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=23ks ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q2MFJ8_STRSD Q2MFJ8_STRSD] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Apramycin biosynthesis features an unusual O-5 phosphorylation on octose-containing pseudotrisaccharide intermediates that functions as a pathway recognition tag. AprI catalyzes 7'-N-methylation of the phosphorylated intermediate. Unexpectedly, sequence and phylogenetic analysis place AprI within the HemK/PrmC superfamily of protein-targeting methyltransferases rather than among canonical small-molecule N-methyltransferases. Here, we report the crystal structures of AprI in apo and SAH-bound forms, revealing a clasp-like homodimer formed by the N-terminal region and a highly dynamic substrate-proximal loop. Structure-guided docking, molecular dynamics simulations, and mutational analysis show that the homodimer forms a composite, positively charged pocket for recognition of the O-5 phosphate tag, whereas the flexible loop positions the 7'-amino group of the pseudotrisaccharide substrate for methyl transfer. Together, these findings establish AprI as an unusual PrmC-like protein methyltransferase repurposed for tailoring phosphorylated natural products, and open opportunities to engineer biocatalysts for site-selective aminoglycoside diversification. | |||
Phosphate-tagged substrate recognition by a PrmC-like methyltransferase in apramycin biosynthesis.,Zhang Q, Zhang Y, Cui Y, Deng Z, Zhao Q, Long F, Yu Y Int J Biol Macromol. 2026 Aug;372:153063. doi: 10.1016/j.ijbiomac.2026.153063. , Epub 2026 Jun 16. PMID:42297172<ref>PMID:42297172</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 23ks" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Streptoalloteichus tenebrarius]] | |||
[[Category: Yu Y]] | |||
[[Category: Zhang Q]] | |||
[[Category: Zhang YX]] | |||
Latest revision as of 07:06, 5 August 2026
Crystal structure of AprI/SAH complex
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