9ylb

Cryo-EM structure of single-loaded human UBA6-UBE2Z/FAT10(a) adenylate complex.

spinqualitylabelsall models 9ylb, resolution 3.24Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9ylb is a 3 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.24Å Ligands: AMP, IHP Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function UBE2Z_HUMAN Catalyzes the covalent attachment of ubiquitin to other proteins (By similarity). Specific substrate for UBA6, not charged with ubiquitin by UBE1. May be involved in apoptosis regulation.[PROSITE-ProRule:PRU00388] Publication Abstract from PubMed UBA1 and UBA6 define parallel ubiquitin (Ub) activation systems that perform non-overlapping roles in Ub and ubiquitin-like protein (Ubl) signaling. Whereas UBA1 supports the canonical Ub pathway, UBA6 also activates the Ubl FAT10, linking Ub signaling to immune-regulated proteostasis. In addition to selective Ub/Ubl activation, UBA1 and UBA6 engage distinct sets of E2s, yet how these enzymes achieve selective E2 engagement has remained unclear. Using chemical trapping and high-resolution cryo-EM, we determine four structures of UBA6-E2 complexes representing the thioester-transfer step with either FAT10 or Ub, revealing how this E1 distinguishes its cognate partners. UBA6 achieves E2 specificity through coordinated contributions of the UFD and SCCH domains, a dual-domain mechanism that contrasts with the UFD-dominated selectivity of UBA1. The structures further show that an existing inositol hexakisphosphate (InsP(6))-binding site, unique to UBA6, stabilizes an expanded SCCH cleft that pre-organizes the enzyme for selective engagement of UBA6-specific E2s. These findings define principles for E1-E2 recognition and identify InsP(6) as a cofactor shaping specificity within the Ub-like conjugation network. Cryo-EM structures of UBA6 reveal mechanisms of E1-E2 specificity and dual FAT10/ubiquitin thioester transfer.,Nayak D, Jia L, Dos Santos Bury P, Ruben EA, Shukla A, Nayak A, Stratton CM, Ebadi P, Cho H, Tumanova AA, Varughese JT, Yuan L, Gao F, Cano KE, Davies C, Sung P, Gack MU, Wasmuth EV, Olsen SK Nat Commun. 2026 Feb 28;17(1):3302. doi: 10.1038/s41467-026-69882-3. PMID:41764162 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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