9uyb: Difference between revisions

Latest revision as of 05:06, 19 August 2026

Crystal structure of ZER1 bound to GSER degron

Structural highlights

9uyb is a 4 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.2Å
Ligands:	GOL
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ZER1_HUMAN Serves as substrate adapter subunit in the E3 ubiquitin ligase complex ZYG11B-CUL2-Elongin BC (PubMed:17304241, PubMed:31273098). Acts to target substrates bearing N-terminal degrons for proteasomal degradation with the first four residues of substrates being the key recognition elements (PubMed:33093214, PubMed:34214466, PubMed:36496439). Involved in the clearance of proteolytic fragments generated by caspase cleavage during apoptosis since N-terminal glycine degrons are strongly enriched at caspase cleavage sites. Also important in the quality control of protein N-myristoylation in which N-terminal glycine degrons are conditionally exposed after a failure of N-myristoylation (PubMed:31273098).^[1] ^[2] ^[3] ^[4] ^[5]

References

↑ Vasudevan S, Starostina NG, Kipreos ET. The Caenorhabditis elegans cell-cycle regulator ZYG-11 defines a conserved family of CUL-2 complex components. EMBO Rep. 2007 Mar;8(3):279-86. Epub 2007 Feb 16. PMID:17304241 doi:https://dx.doi.org/7400895
↑ Timms RT, Zhang Z, Rhee DY, Harper JW, Koren I, Elledge SJ. A glycine-specific N-degron pathway mediates the quality control of protein N-myristoylation. Science. 2019 Jul 5;365(6448):eaaw4912. doi: 10.1126/science.aaw4912. PMID:31273098 doi:https://dx.doi.org/10.1126/science.aaw4912
↑ Robinson KS, Teo DET, Tan KS, Toh GA, Ong HH, Lim CK, Lay K, Au BV, Lew TS, Chu JJH, Chow VTK, Wang Y, Zhong FL, Reversade B. Enteroviral 3C protease activates the human NLRP1 inflammasome in airway epithelia. Science. 2020 Dec 4;370(6521):eaay2002. doi: 10.1126/science.aay2002. Epub 2020 , Oct 22. PMID:33093214 doi:https://dx.doi.org/10.1126/science.aay2002
↑ Yan X, Li Y, Wang G, Zhou Z, Song G, Feng Q, Zhao Y, Mi W, Ma Z, Dong C. Molecular basis for recognition of Gly/N-degrons by CRL2(ZYG11B) and CRL2(ZER1). Mol Cell. 2021 Aug 19;81(16):3262-3274.e3. PMID:34214466 doi:10.1016/j.molcel.2021.06.010
↑ Li Y, Zhao Y, Yan X, Ye C, Weirich S, Zhang B, Wang X, Song L, Jiang C, Jeltsch A, Dong C, Mi W. CRL2(ZER1/ZYG11B) recognizes small N-terminal residues for degradation. Nat Commun. 2022 Dec 10;13(1):7636. doi: 10.1038/s41467-022-35169-6. PMID:36496439 doi:https://dx.doi.org/10.1038/s41467-022-35169-6

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Vasudevan S, Starostina NG, Kipreos ET. The Caenorhabditis elegans cell-cycle regulator ZYG-11 defines a conserved family of CUL-2 complex components. EMBO Rep. 2007 Mar;8(3):279-86. Epub 2007 Feb 16. PMID:17304241 doi:https://dx.doi.org/7400895

[2] Timms RT, Zhang Z, Rhee DY, Harper JW, Koren I, Elledge SJ. A glycine-specific N-degron pathway mediates the quality control of protein N-myristoylation. Science. 2019 Jul 5;365(6448):eaaw4912. doi: 10.1126/science.aaw4912. PMID:31273098 doi:https://dx.doi.org/10.1126/science.aaw4912

[3] Robinson KS, Teo DET, Tan KS, Toh GA, Ong HH, Lim CK, Lay K, Au BV, Lew TS, Chu JJH, Chow VTK, Wang Y, Zhong FL, Reversade B. Enteroviral 3C protease activates the human NLRP1 inflammasome in airway epithelia. Science. 2020 Dec 4;370(6521):eaay2002. doi: 10.1126/science.aay2002. Epub 2020 , Oct 22. PMID:33093214 doi:https://dx.doi.org/10.1126/science.aay2002

[4] Yan X, Li Y, Wang G, Zhou Z, Song G, Feng Q, Zhao Y, Mi W, Ma Z, Dong C. Molecular basis for recognition of Gly/N-degrons by CRL2(ZYG11B) and CRL2(ZER1). Mol Cell. 2021 Aug 19;81(16):3262-3274.e3. PMID:34214466 doi:10.1016/j.molcel.2021.06.010

[5] Li Y, Zhao Y, Yan X, Ye C, Weirich S, Zhang B, Wang X, Song L, Jiang C, Jeltsch A, Dong C, Mi W. CRL2(ZER1/ZYG11B) recognizes small N-terminal residues for degradation. Nat Commun. 2022 Dec 10;13(1):7636. doi: 10.1038/s41467-022-35169-6. PMID:36496439 doi:https://dx.doi.org/10.1038/s41467-022-35169-6

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 9uyb is ON HOLD  until 2027-08-15
+==Crystal structure of ZER1 bound to GSER degron==
+<StructureSection load='9uyb' size='340' side='right'caption='[[9uyb]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
-Authors: Dong, C., Wang, L., Zhang, B., Li, J.
+== Structural highlights ==
+<table><tr><td colspan='2'>[[9uyb]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9UYB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9UYB FirstGlance]. <br>
-Description: Crystal structure of ZER1 bound to GSER degron
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
-[[Category: Unreleased Structures]]
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-[[Category: Wang, L]]
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9uyb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9uyb OCA], [https://pdbe.org/9uyb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9uyb RCSB], [https://www.ebi.ac.uk/pdbsum/9uyb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9uyb ProSAT]</span></td></tr>
-[[Category: Li, J]]
+</table>
-[[Category: Zhang, B]]
+== Function ==
-[[Category: Dong, C]]
+[https://www.uniprot.org/uniprot/ZER1_HUMAN ZER1_HUMAN] Serves as substrate adapter subunit in the E3 ubiquitin ligase complex ZYG11B-CUL2-Elongin BC (PubMed:17304241, PubMed:31273098). Acts to target substrates bearing N-terminal degrons for proteasomal degradation with the first four residues of substrates being the key recognition elements (PubMed:33093214, PubMed:34214466, PubMed:36496439). Involved in the clearance of proteolytic fragments generated by caspase cleavage during apoptosis since N-terminal glycine degrons are strongly enriched at caspase cleavage sites. Also important in the quality control of protein N-myristoylation in which N-terminal glycine degrons are conditionally exposed after a failure of N-myristoylation (PubMed:31273098).<ref>PMID:17304241</ref> <ref>PMID:31273098</ref> <ref>PMID:33093214</ref> <ref>PMID:34214466</ref> <ref>PMID:36496439</ref>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Homo sapiens]]
+[[Category: Large Structures]]
+[[Category: Dong C]]
+[[Category: Li J]]
+[[Category: Wang L]]
+[[Category: Zhang B]]

9uyb: Difference between revisions

Latest revision as of 05:06, 19 August 2026

Crystal structure of ZER1 bound to GSER degron

Structural highlights

Function

References

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