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| <StructureSection load='' size='350' side='right' caption='Pyranose oxidase complex with FAD and deoxy-fluoro-glucopyranose (PDB code [[3k4l]])' scene='70/708805/Cv/1'> | | <StructureSection load='' size='350' side='right' caption='Pyranose oxidase complex with FAD and deoxy-fluoro-glucopyranose (PDB code [[3k4l]])' scene='70/708805/Cv/1'> |
| == Function == | | == Function == |
| '''Pyranose oxidase''' or '''pyranose 2-oxidase''' (P2O) catalyzes the conversion of D-glucose and molecular oxygen to 2-dehydro-D-glucose and H<sub>2</sub>O<sub>2</sub>. It is abundant in lignin-degrading white rot fungi. P2O is a <scene name='70/708805/Cv/7'>tetramer</scene> flavoprotein containing the <scene name='70/708805/Cv/8'>prosthetic group FAD in each monomer</scene>. P2O oxidizes several aldopyranoses with the preferred electron donors being D-glucose, D-xylose and D-sorbose<ref>PMID:11152063</ref>. | | '''Pyranose oxidase''' or '''pyranose 2-oxidase''' (P2O) or '''C-glucosyl oxidoreductase''' catalyzes the conversion of D-glucose and molecular oxygen to 2-dehydro-D-glucose and H<sub>2</sub>O<sub>2</sub>. It is abundant in lignin-degrading white rot fungi. P2O is a <scene name='70/708805/Cv/7'>tetramer</scene> flavoprotein containing the <scene name='70/708805/Cv/8'>prosthetic group FAD in each monomer</scene>. P2O oxidizes several aldopyranoses with the preferred electron donors being D-glucose, D-xylose and D-sorbose<ref>PMID:11152063</ref>. |
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| == Relevance == | | == Relevance == |
Revision as of 07:29, 23 August 2026
| Function
Pyranose oxidase or pyranose 2-oxidase (P2O) or C-glucosyl oxidoreductase catalyzes the conversion of D-glucose and molecular oxygen to 2-dehydro-D-glucose and H2O2. It is abundant in lignin-degrading white rot fungi. P2O is a tetramer flavoprotein containing the prosthetic group FAD in each monomer. P2O oxidizes several aldopyranoses with the preferred electron donors being D-glucose, D-xylose and D-sorbose[1].
Relevance
Converting common sugars and sugar derivatives with P2O provides a pool of sugar-derived intermediates for the synthesis of rare sugars, fine chemicals and drugs.
Structural highlights
The active site of P2O is gated by a highly conserved loop which determines the substrate specificity. Water molecules are shown as red spheres. The active site contains the FAD cofactor[2].
- ↑ Giffhorn F. Fungal pyranose oxidases: occurrence, properties and biotechnical applications in carbohydrate chemistry. Appl Microbiol Biotechnol. 2000 Dec;54(6):727-40. PMID:11152063
- ↑ Spadiut O, Tan TC, Pisanelli I, Haltrich D, Divne C. Importance of the gating segment in the substrate-recognition loop of pyranose 2-oxidase. FEBS J. 2010 Jul;277(13):2892-909. Epub 2010 Jun 2. PMID:20528921 doi:10.1111/j.1742-4658.2010.07705.x
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3D Structures of pyranose oxidase
Updated on 23-August-2026
{"openlevels":0}
- Pyranose oxidase
- 1tzl – PeP2O + FAD – Peniophora
- 2f6c – PeP2O (mutant) + FAD
- 3bg6, 3bg7, 3bly, 3fdy – TmP2O (mutant) + FAD – Trametes multicolor
- 1tt0, 2igk – ToP2O + FAD – Trametes ochracea
- 2igm, 2ign, 3k4b, 3k4c, 3k4j, 3k4k, 3k4n, 3lsh, 3lsi, 3lsk, 4mok – ToP2O (mutant) + FAD
- 4mif, 4mig – ToP2O + FAD derivative
- 3lsm – ToP2O (mutant) + FAD derivative
- Pyranose oxidase complex
- 2f5v – PeP2O (mutant) + FAD + keto-glucose
- 2igo, 3k4l, 3k4m, 4mih – ToP2O (mutant) + FAD + deoxy-fluoro-glucopyranose
- 4moe, 4mof, 4mog, 4moh, 4moi, 4moj, 4mol – ToP2O (mutant) + FAD derivative + deoxy-fluoro-glucopyranose
- 4mom, 4mop, 4moq, 4mor – ToP2O (mutant) + FAD derivative + deoxy-fluoro-galactopyranose
- 4moo – ToP2O (mutant) + FAD derivative + deoxy-fluoro-galactose
- 4mos – ToP2O (mutant) + FAD derivative + deoxy-fluoro-galactopyranose + deoxy-fluoro-galactose
- 3pl8 – ToP2O (mutant) + FAD + deoxy-fluoro-glucose
References
