11it: Difference between revisions
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==1332E5-RBD KP3.1.1 Complex Local Refine== | |||
<StructureSection load='11it' size='340' side='right'caption='[[11it]], [[Resolution|resolution]] 3.62Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[11it]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=11IT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=11IT FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.62Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=11it FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=11it OCA], [https://pdbe.org/11it PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=11it RCSB], [https://www.ebi.ac.uk/pdbsum/11it PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=11it ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) evolution reduces the efficacy of prophylactic vaccines and monoclonal antibody (mAb) therapies. To evaluate potency and breadth, receptor binding domain (RBD)-targeting neutralizing mAbs were identified from patient B cells using an Omicron KP3.1.1 Spike (S) protein bait. MAbs exhibiting the greatest neutralization potency (1332D4 and 1332E5) and binding breadth (1324A10 and 1316C10) were evaluated in greater detail. Cryo-electron microscopy (Cryo-EM) studies revealed 1332D4 and 1332E5 target RBD residue segments 439-446 and 498-506 of KP3.1.1 S, respectively. 1332E5 is a knob(498-506)-targeting class-1/4 mAb that exhibits pan-Omicron specificity but does not bind or neutralize ancestral Wuhan-1. Further analysis suggests mAbs generically defined as class 1/4 mAbs may be separated into two classes. Class 1/4 mAbs, like 1332E5, and class 4/1 mAbs that make extensive interactions with the class-4 epitope and limited contacts with the class-1 knob(498-596). Despite these differences, the specificity of both mAb classes can be altered by mutations in knob(498-506). | |||
Emergence of neutralizing RBD antibodies following Omicron infection with limited activity against ancestral SARS-CoV-2.,Piepenbrink MS, Ma Y, Panjwani S, Blake AR, Bell AM, Kizziah JL, Mahmoud SH, Ippolito GC, Erdmann NB, Goepfert PA, Martinez-Sobrido L, Kobie JJ, Walter MR iScience. 2026 Aug 11;29(8):117166. doi: 10.1016/j.isci.2026.117166. eCollection , 2026 Aug 21. PMID:42621159<ref>PMID:42621159</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 11it" style="background-color:#fffaf0;"></div> | ||
[[Category: Kizziah | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Severe acute respiratory syndrome coronavirus 2]] | |||
[[Category: Kizziah JL]] | |||
[[Category: Walter MR]] | |||
Latest revision as of 16:57, 8 September 2026
1332E5-RBD KP3.1.1 Complex Local Refine
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