4b09: Difference between revisions

Latest revision as of 17:57, 8 September 2026

Structure of unphosphorylated BaeR dimer

Structural highlights

4b09 is a 12 chain structure with sequence from Escherichia coli str. K-12 substr. MG1655. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.3Å
Ligands:	CAS
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

BAER_ECOLI Member of the two-component regulatory system BaeS/BaeR which responds to envelope stress (PubMed:12354228). Activates expression of periplasmic chaperone spy in response to spheroplast formation, indole and P pili protein PapG overexpression (PubMed:12354228). Activates the mdtABCD (PubMed:12107133, PubMed:12107134) and probably the CRISPR-Cas casABCDE-ygbT-ygbF operon (PubMed:21255106).^[1] ^[2] ^[3] ^[4]

Publication Abstract from PubMed

Bacterial response regulators can regulate the expression of genes that confer antibiotic resistance; they contain a receiver and an effector domain and their ability to bind DNA is based on the dimerization state. This is triggered by phosphorylation of the receiver domain by a kinase. However, even in the absence of phosphorylation response regulators can exist in equilibrium between monomers and dimers with phosphorylation shifting the equilibrium towards the dimer form. We have determined the crystal structure of the unphosphorylated dimeric BaeR from Escherichia coli. The dimer interface is formed by a domain swap at the receiver domain. In comparison with the unphosphorylated dimeric PhoP from Mycobacterium tuberculosis, BaeR displays an asymmetry of the effector domains.

The dimeric form of the unphosphorylated response regulator BaeR.,Choudhury HG, Beis K Protein Sci. 2013 Jul 19. doi: 10.1002/pro.2311. PMID:23868292^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Nagakubo S, Nishino K, Hirata T, Yamaguchi A. The putative response regulator BaeR stimulates multidrug resistance of Escherichia coli via a novel multidrug exporter system, MdtABC. J Bacteriol. 2002 Aug;184(15):4161-7. doi: 10.1128/JB.184.15.4161-4167.2002. PMID:12107133 doi:https://dx.doi.org/10.1128/JB.184.15.4161-4167.2002
↑ Baranova N, Nikaido H. The baeSR two-component regulatory system activates transcription of the yegMNOB (mdtABCD) transporter gene cluster in Escherichia coli and increases its resistance to novobiocin and deoxycholate. J Bacteriol. 2002 Aug;184(15):4168-76. doi: 10.1128/JB.184.15.4168-4176.2002. PMID:12107134 doi:https://dx.doi.org/10.1128/JB.184.15.4168-4176.2002
↑ Raffa RG, Raivio TL. A third envelope stress signal transduction pathway in Escherichia coli. Mol Microbiol. 2002 Sep;45(6):1599-611. doi: 10.1046/j.1365-2958.2002.03112.x. PMID:12354228 doi:https://dx.doi.org/10.1046/j.1365-2958.2002.03112.x
↑ Perez-Rodriguez R, Haitjema C, Huang Q, Nam KH, Bernardis S, Ke A, DeLisa MP. Envelope stress is a trigger of CRISPR RNA-mediated DNA silencing in Escherichia coli. Mol Microbiol. 2011 Feb;79(3):584-99. doi: 10.1111/j.1365-2958.2010.07482.x. Epub, 2010 Dec 13. PMID:21255106 doi:10.1111/j.1365-2958.2010.07482.x
↑ Choudhury HG, Beis K. The dimeric form of the unphosphorylated response regulator BaeR. Protein Sci. 2013 Jul 19. doi: 10.1002/pro.2311. PMID:23868292 doi:10.1002/pro.2311

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Nagakubo S, Nishino K, Hirata T, Yamaguchi A. The putative response regulator BaeR stimulates multidrug resistance of Escherichia coli via a novel multidrug exporter system, MdtABC. J Bacteriol. 2002 Aug;184(15):4161-7. doi: 10.1128/JB.184.15.4161-4167.2002. PMID:12107133 doi:https://dx.doi.org/10.1128/JB.184.15.4161-4167.2002

[2] Baranova N, Nikaido H. The baeSR two-component regulatory system activates transcription of the yegMNOB (mdtABCD) transporter gene cluster in Escherichia coli and increases its resistance to novobiocin and deoxycholate. J Bacteriol. 2002 Aug;184(15):4168-76. doi: 10.1128/JB.184.15.4168-4176.2002. PMID:12107134 doi:https://dx.doi.org/10.1128/JB.184.15.4168-4176.2002

[3] Raffa RG, Raivio TL. A third envelope stress signal transduction pathway in Escherichia coli. Mol Microbiol. 2002 Sep;45(6):1599-611. doi: 10.1046/j.1365-2958.2002.03112.x. PMID:12354228 doi:https://dx.doi.org/10.1046/j.1365-2958.2002.03112.x

[4] Perez-Rodriguez R, Haitjema C, Huang Q, Nam KH, Bernardis S, Ke A, DeLisa MP. Envelope stress is a trigger of CRISPR RNA-mediated DNA silencing in Escherichia coli. Mol Microbiol. 2011 Feb;79(3):584-99. doi: 10.1111/j.1365-2958.2010.07482.x. Epub, 2010 Dec 13. PMID:21255106 doi:10.1111/j.1365-2958.2010.07482.x

[5] Choudhury HG, Beis K. The dimeric form of the unphosphorylated response regulator BaeR. Protein Sci. 2013 Jul 19. doi: 10.1002/pro.2311. PMID:23868292 doi:10.1002/pro.2311

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[2]

[3]

[4]

[5]

@@ Line 3: / Line 3: @@
 <StructureSection load='4b09' size='340' side='right'caption='[[4b09]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4b09]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_mg1655 Escherichia coli mg1655]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B09 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4B09 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4b09]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_str._K-12_substr._MG1655 Escherichia coli str. K-12 substr. MG1655]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B09 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4B09 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TBR:HEXATANTALUM+DODECABROMIDE'>TBR</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
-<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CAS:S-(DIMETHYLARSENIC)CYSTEINE'>CAS</scene></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CAS:S-(DIMETHYLARSENIC)CYSTEINE'>CAS</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4b09 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b09 OCA], [https://pdbe.org/4b09 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4b09 RCSB], [https://www.ebi.ac.uk/pdbsum/4b09 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4b09 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/BAER_ECOL6 BAER_ECOL6]] Member of the two-component regulatory system BaeS/BaeR. Activates the mdtABCD operon (By similarity).
+[https://www.uniprot.org/uniprot/BAER_ECOLI BAER_ECOLI] Member of the two-component regulatory system BaeS/BaeR which responds to envelope stress (PubMed:12354228). Activates expression of periplasmic chaperone spy in response to spheroplast formation, indole and P pili protein PapG overexpression (PubMed:12354228). Activates the mdtABCD (PubMed:12107133, PubMed:12107134) and probably the CRISPR-Cas casABCDE-ygbT-ygbF operon (PubMed:21255106).<ref>PMID:12107133</ref> <ref>PMID:12107134</ref> <ref>PMID:12354228</ref> <ref>PMID:21255106</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Escherichia coli mg1655]]
+[[Category: Escherichia coli str. K-12 substr. MG1655]]
 [[Category: Large Structures]]
-[[Category: Beis, K]]
+[[Category: Beis K]]
-[[Category: Choudhury, H]]
+[[Category: Choudhury H]]
-[[Category: Dna binding]]
-[[Category: Response regulator]]
-[[Category: Transcription]]

4b09: Difference between revisions

Latest revision as of 17:57, 8 September 2026

Structure of unphosphorylated BaeR dimer

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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