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| == Function == | | == Function == |
| [https://www.uniprot.org/uniprot/V5QR23_BETVV V5QR23_BETVV] | | [https://www.uniprot.org/uniprot/V5QR23_BETVV V5QR23_BETVV] |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| Phytoglobins (Pgbs) are plant-originating heme proteins of the globin superfamily with varying degrees of hexacoordination. Pgbs have a conserved cysteine residue, the role of which is poorly understood. In this paper, we investigated the functional and structural role of cysteine in BvPgb1.2, a Class 1 Pgb from sugar beet (Beta vulgaris), by constructing an alanine-substituted mutant (Cys86Ala). The substitution had little impact on structure, dimerization, and heme loss as determined by X-ray crystallography, size-exclusion chromatography, and an apomyoglobin-based heme-loss assay, respectively. The substitution significantly affected other important biochemical properties. The autoxidation rate increased 16.7- and 14.4-fold for the mutant versus the native protein at 25 degrees C and 37 degrees C, respectively. Thermal stability similarly increased for the mutant by ~2.5 degrees C as measured by nano-differential scanning fluorimetry. Monitoring peroxidase activity over 7 days showed a 60% activity decrease in the native protein, from 33.7 to 20.2 U/mg protein. When comparing the two proteins, the mutant displayed a remarkable enzymatic stability as activity remained relatively constant throughout, albeit at a lower level, ~12 U/mg protein. This suggests that cysteine plays an important role in BvPgb1.2 function and stability, despite having seemingly little effect on its tertiary and quaternary structure.
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| Oxidative Implications of Substituting a Conserved Cysteine Residue in Sugar Beet Phytoglobin BvPgb 1.2.,Christensen S, Groth L, Leiva-Eriksson N, Nyblom M, Bulow L Antioxidants (Basel). 2022 Aug 20;11(8). pii: antiox11081615. doi:, 10.3390/antiox11081615. PMID:36009334<ref>PMID:36009334</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 7z1u" style="background-color:#fffaf0;"></div>
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| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
