9tiu: Difference between revisions

Latest revision as of 08:06, 9 September 2026

cryo-EM structure of wild-type MscL from Escherichia coli in MSP nanodisc

Structural highlights

9tiu is a 5 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.68Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MSCL_ECOLI Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. Forms a nonselective ion channel with a conductance of about 4 nanosiemens. Participates in the regulation of osmotic pressure changes within the cell. Opens at a pressure just below that which would cause cell disruption and death. The force required to trigger channel opening depends on the membrane lipids composition.^[1] ^[2] ^[3] ^[4] ^[5]

References

↑ Levina N, Totemeyer S, Stokes NR, Louis P, Jones MA, Booth IR. Protection of Escherichia coli cells against extreme turgor by activation of MscS and MscL mechanosensitive channels: identification of genes required for MscS activity. EMBO J. 1999 Apr 1;18(7):1730-7. PMID:10202137 doi:https://dx.doi.org/10.1093/emboj/18.7.1730
↑ Yang LM, Zhong D, Blount P. Chimeras reveal a single lipid-interface residue that controls MscL channel kinetics as well as mechanosensitivity. Cell Rep. 2013 Feb 21;3(2):520-7. doi: 10.1016/j.celrep.2013.01.018. Epub 2013 , Feb 14. PMID:23416054 doi:https://dx.doi.org/10.1016/j.celrep.2013.01.018
↑ Zhong D, Blount P. Phosphatidylinositol is crucial for the mechanosensitivity of Mycobacterium tuberculosis MscL. Biochemistry. 2013 Aug 13;52(32):5415-20. doi: 10.1021/bi400790j. Epub 2013 Aug , 1. PMID:23875651 doi:https://dx.doi.org/10.1021/bi400790j
↑ Sukharev SI, Blount P, Martinac B, Blattner FR, Kung C. A large-conductance mechanosensitive channel in E. coli encoded by mscL alone. Nature. 1994 Mar 17;368(6468):265-8. PMID:7511799 doi:https://dx.doi.org/10.1038/368265a0
↑ Moe PC, Blount P, Kung C. Functional and structural conservation in the mechanosensitive channel MscL implicates elements crucial for mechanosensation. Mol Microbiol. 1998 May;28(3):583-92. doi: 10.1046/j.1365-2958.1998.00821.x. PMID:9632260 doi:https://dx.doi.org/10.1046/j.1365-2958.1998.00821.x

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OCA

[1] Levina N, Totemeyer S, Stokes NR, Louis P, Jones MA, Booth IR. Protection of Escherichia coli cells against extreme turgor by activation of MscS and MscL mechanosensitive channels: identification of genes required for MscS activity. EMBO J. 1999 Apr 1;18(7):1730-7. PMID:10202137 doi:https://dx.doi.org/10.1093/emboj/18.7.1730

[2] Yang LM, Zhong D, Blount P. Chimeras reveal a single lipid-interface residue that controls MscL channel kinetics as well as mechanosensitivity. Cell Rep. 2013 Feb 21;3(2):520-7. doi: 10.1016/j.celrep.2013.01.018. Epub 2013 , Feb 14. PMID:23416054 doi:https://dx.doi.org/10.1016/j.celrep.2013.01.018

[3] Zhong D, Blount P. Phosphatidylinositol is crucial for the mechanosensitivity of Mycobacterium tuberculosis MscL. Biochemistry. 2013 Aug 13;52(32):5415-20. doi: 10.1021/bi400790j. Epub 2013 Aug , 1. PMID:23875651 doi:https://dx.doi.org/10.1021/bi400790j

[4] Sukharev SI, Blount P, Martinac B, Blattner FR, Kung C. A large-conductance mechanosensitive channel in E. coli encoded by mscL alone. Nature. 1994 Mar 17;368(6468):265-8. PMID:7511799 doi:https://dx.doi.org/10.1038/368265a0

[5] Moe PC, Blount P, Kung C. Functional and structural conservation in the mechanosensitive channel MscL implicates elements crucial for mechanosensation. Mol Microbiol. 1998 May;28(3):583-92. doi: 10.1046/j.1365-2958.1998.00821.x. PMID:9632260 doi:https://dx.doi.org/10.1046/j.1365-2958.1998.00821.x

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 9tiu is ON HOLD  until Paper Publication
+==cryo-EM structure of wild-type MscL from Escherichia coli in MSP nanodisc==
+<StructureSection load='9tiu' size='340' side='right'caption='[[9tiu]], [[Resolution|resolution]] 3.68&Aring;' scene=''>
-Authors:
+== Structural highlights ==
+<table><tr><td colspan='2'>[[9tiu]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9TIU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9TIU FirstGlance]. <br>
-Description:
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.68&#8491;</td></tr>
-[[Category: Unreleased Structures]]
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9tiu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9tiu OCA], [https://pdbe.org/9tiu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9tiu RCSB], [https://www.ebi.ac.uk/pdbsum/9tiu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9tiu ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/MSCL_ECOLI MSCL_ECOLI] Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. Forms a nonselective ion channel with a conductance of about 4 nanosiemens. Participates in the regulation of osmotic pressure changes within the cell. Opens at a pressure just below that which would cause cell disruption and death. The force required to trigger channel opening depends on the membrane lipids composition.<ref>PMID:10202137</ref> <ref>PMID:23416054</ref> <ref>PMID:23875651</ref> <ref>PMID:7511799</ref> <ref>PMID:9632260</ref>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Escherichia coli]]
+[[Category: Large Structures]]
+[[Category: Lange A]]
+[[Category: Sprink T]]
+[[Category: Xiao T]]

9tiu: Difference between revisions

Latest revision as of 08:06, 9 September 2026

cryo-EM structure of wild-type MscL from Escherichia coli in MSP nanodisc

Structural highlights

Function

References

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