9wn6: Difference between revisions

Line 1:

~~'''Unreleased structure'''~~

~~The entry~~ 9wn6 is ~~ON HOLD until Paper Publication~~

==HNHNme1-AcrIIC1BoeST==

~~Authors~~: ~~Wang~~, S., ~~Xiao~~, Y.J.

== Structural highlights ==

<table><tr><td colspan='2'>[[9wn6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_meningitidis_Z2491 Neisseria meningitidis Z2491]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9WN6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9WN6 FirstGlance]. <br>

~~Description~~: ~~HNHNme1~~-~~AcrIIC1BoeST~~

</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr>

[[Category: ~~Unreleased~~ Structures]]

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9wn6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9wn6 OCA], [https://pdbe.org/9wn6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9wn6 RCSB], [https://www.ebi.ac.uk/pdbsum/9wn6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9wn6 ProSAT]</span></td></tr>

[[Category: ~~Xiao, Y.J~~]]

</table>

[[Category: Wang, S]]

== Function ==

[https://www.uniprot.org/uniprot/CAS9_NEIMA CAS9_NEIMA] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity (By similarity). Cuts target DNA in Cas9:gRNAs mixing experiments with C.jejuni strain NCTC 11168 and P.multocoda strain Pm70.[HAMAP-Rule:MF_01480]<ref>PMID:24270795</ref>

== References ==

__TOC__

</StructureSection>

[[Category: Large Structures]]

[[Category: Neisseria meningitidis Z2491]]

[[Category: Wang S]]

[[Category: Xiao YJ]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 9wn6 is ON HOLD  until Paper Publication
+==HNHNme1-AcrIIC1BoeST==
+<StructureSection load='9wn6' size='340' side='right'caption='[[9wn6]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
-Authors: Wang, S., Xiao, Y.J.
+== Structural highlights ==
+<table><tr><td colspan='2'>[[9wn6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_meningitidis_Z2491 Neisseria meningitidis Z2491]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9WN6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9WN6 FirstGlance]. <br>
-Description: HNHNme1-AcrIIC1BoeST
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
-[[Category: Unreleased Structures]]
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9wn6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9wn6 OCA], [https://pdbe.org/9wn6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9wn6 RCSB], [https://www.ebi.ac.uk/pdbsum/9wn6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9wn6 ProSAT]</span></td></tr>
-[[Category: Xiao, Y.J]]
+</table>
-[[Category: Wang, S]]
+== Function ==
+[https://www.uniprot.org/uniprot/CAS9_NEIMA CAS9_NEIMA] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity (By similarity). Cuts target DNA in Cas9:gRNAs mixing experiments with C.jejuni strain NCTC 11168 and P.multocoda strain Pm70.[HAMAP-Rule:MF_01480]<ref>PMID:24270795</ref>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Neisseria meningitidis Z2491]]
+[[Category: Wang S]]
+[[Category: Xiao YJ]]