11vq: Difference between revisions

Line 1:

~~'''Unreleased structure'''~~

~~The entry~~ 11vq is ~~ON HOLD until Paper Publication~~

==DNA-guided AsCas12a==

~~Authors~~:

== Structural highlights ==

<table><tr><td colspan='2'>[[11vq]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Unclassified_Acidaminococcus Unclassified Acidaminococcus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=11VQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=11VQ FirstGlance]. <br>

~~Description~~:

</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.18Å</td></tr>

[[Category: ~~Unreleased~~ Structures]]

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=11vq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=11vq OCA], [https://pdbe.org/11vq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=11vq RCSB], [https://www.ebi.ac.uk/pdbsum/11vq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=11vq ProSAT]</span></td></tr>

</table>

== Function ==

[https://www.uniprot.org/uniprot/CS12A_ACISB CS12A_ACISB] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Recognizes a short motif in the CRISPR repeat sequences (the 5' PAM or protospacer adjacent motif, TTTN in this organism) to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs (PubMed:26422227). Has dsDNA endonuclease activity, results in staggered 4-base 5' overhangs 19 and 22 bases downstream of the PAM on the non-targeted and targeted strand respectively (PubMed:26422227). Non-target strand cleavage by the RuvC domain is probably a prerequisite of target strand cleavage by the Nuc domain (PubMed:27114038). In this CRISPR system correct processing of pre-crRNA requires only this protein and the CRISPR locus (By similarity).[UniProtKB:A0Q7Q2]<ref>PMID:26422227</ref> <ref>PMID:27114038</ref>

== References ==

__TOC__

</StructureSection>

[[Category: Large Structures]]

[[Category: Unclassified Acidaminococcus]]

[[Category: Fregoso Ocampo R]]

[[Category: Jain PK]]

[[Category: Orosco C]]

[[Category: Taylor DW]]

[[Category: West MW]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 11vq is ON HOLD  until Paper Publication
+==DNA-guided AsCas12a==
+<StructureSection load='11vq' size='340' side='right'caption='[[11vq]], [[Resolution|resolution]] 3.18&Aring;' scene=''>
-Authors:
+== Structural highlights ==
+<table><tr><td colspan='2'>[[11vq]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Unclassified_Acidaminococcus Unclassified Acidaminococcus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=11VQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=11VQ FirstGlance]. <br>
-Description:
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.18&#8491;</td></tr>
-[[Category: Unreleased Structures]]
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=11vq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=11vq OCA], [https://pdbe.org/11vq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=11vq RCSB], [https://www.ebi.ac.uk/pdbsum/11vq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=11vq ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/CS12A_ACISB CS12A_ACISB] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Recognizes a short motif in the CRISPR repeat sequences (the 5' PAM or protospacer adjacent motif, TTTN in this organism) to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs (PubMed:26422227). Has dsDNA endonuclease activity, results in staggered 4-base 5' overhangs 19 and 22 bases downstream of the PAM on the non-targeted and targeted strand respectively (PubMed:26422227). Non-target strand cleavage by the RuvC domain is probably a prerequisite of target strand cleavage by the Nuc domain (PubMed:27114038). In this CRISPR system correct processing of pre-crRNA requires only this protein and the CRISPR locus (By similarity).[UniProtKB:A0Q7Q2]<ref>PMID:26422227</ref> <ref>PMID:27114038</ref>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Unclassified Acidaminococcus]]
+[[Category: Fregoso Ocampo R]]
+[[Category: Jain PK]]
+[[Category: Orosco C]]
+[[Category: Taylor DW]]
+[[Category: West MW]]