9vt1: Difference between revisions

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'''Unreleased structure'''


The entry 9vt1 is ON HOLD  until Paper Publication
==Structure of Cdr1 with ATP/ADP==
<StructureSection load='9vt1' size='340' side='right'caption='[[9vt1]], [[Resolution|resolution]] 2.46&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9vt1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans_SC5314 Candida albicans SC5314]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9VT1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9VT1 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.46&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9vt1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9vt1 OCA], [https://pdbe.org/9vt1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9vt1 RCSB], [https://www.ebi.ac.uk/pdbsum/9vt1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9vt1 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CDR1_CANAL CDR1_CANAL] Pleiotropic ABC efflux transporter that confers resistance to numerous chemicals including anisomycin, cycloheximide, fluconazole, miconazole, ketoconazole, itriconazole, nystatin, terbinafine, amorolfine, brefeldin A, amphotericin B, fluphenazine, as well as estrogen. Plays a role in farnesol-induced apoptotic process through glutathione efflux activity. Mediates in-to-out translocation of membrane phospholipids including aminophospholipids and thus regulates asymmetric distribution of phosphatidylethanolamine. Exhibits nucleoside triphosphatase activity.<ref>PMID:10029989</ref> <ref>PMID:10227177</ref> <ref>PMID:10428921</ref> <ref>PMID:11709310</ref> <ref>PMID:11870854</ref> <ref>PMID:12709320</ref> <ref>PMID:14665469</ref> <ref>PMID:15152937</ref> <ref>PMID:15190023</ref> <ref>PMID:15486081</ref> <ref>PMID:15937063</ref> <ref>PMID:16291868</ref> <ref>PMID:16475832</ref> <ref>PMID:16622073</ref> <ref>PMID:16782311</ref> <ref>PMID:17202662</ref> <ref>PMID:18056285</ref> <ref>PMID:18268086</ref> <ref>PMID:18710914</ref> <ref>PMID:19223631</ref> <ref>PMID:19393219</ref> <ref>PMID:20002912</ref> <ref>PMID:20348384</ref> <ref>PMID:20876623</ref> <ref>PMID:22166216</ref> <ref>PMID:22205973</ref> <ref>PMID:23824183</ref> <ref>PMID:23979757</ref> <ref>PMID:8891134</ref> <ref>PMID:9124851</ref> <ref>PMID:9453158</ref> <ref>PMID:9605504</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In Candida albicans-a World Health Organization fungal priority pathogen-overexpression of the adenosine triphosphate (ATP)-binding cassette transporter Cdr1 drives multidrug resistance. We present seven cryo-electron microscopy structures capturing substrate entry and expulsion. An inward-facing transmembrane channel with three on-off substrate binding sites defines a proposed entry pathway for a single substrate molecule. Coordinated ATP binding to both nucleotide-binding domains induces transmembrane domain closure, driving the substrate expulsion; adenosine diphosphate release following ATP hydrolysis resets the transporter to an inward-open conformation, enabling substrate entry for the next translocation cycle. Structures with three structurally diverse inhibitors resolve two distinct binding modes: one occupying all three substrate sites and another specifically binding two extracellular-proximal sites. These findings provide snapshots of the substrate translocation cycle and structural blueprints for antifungal drug design.


Authors:  
Cryo-EM structures of Cdr1 reveal snapshots of substrate transport and diverse inhibitor recognition.,Wang Z, Yang S, Zhang B, Jiang H, Li Y, Gao R, Wang Y, Fan F, Dong L, Qiu J, Li X, Zhou Y, Murchie AIH, Yu X Sci Adv. 2026 Aug 28;12(35):eaef7706. doi: 10.1126/sciadv.aef7706. Epub 2026 Aug , 28. PMID:42664345<ref>PMID:42664345</ref>


Description:  
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
<div class="pdbe-citations 9vt1" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Candida albicans SC5314]]
[[Category: Large Structures]]
[[Category: Wang Z]]
[[Category: Yang S]]
[[Category: Yu X]]
[[Category: Zhang B]]
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