9srh: Difference between revisions
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==helical form of Polybia-CP== | |||
<StructureSection load='9srh' size='340' side='right'caption='[[9srh]], [[Resolution|resolution]] 1.13Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9srh]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Polybia_paulista Polybia paulista]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9SRH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9SRH FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.13Å</td></tr> | |||
[[Category: | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TFA:TRIFLUOROACETIC+ACID'>TFA</scene></td></tr> | ||
[[Category: Bloch | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9srh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9srh OCA], [https://pdbe.org/9srh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9srh RCSB], [https://www.ebi.ac.uk/pdbsum/9srh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9srh ProSAT]</span></td></tr> | ||
[[Category: | </table> | ||
[[Category: | == Function == | ||
[https://www.uniprot.org/uniprot/PROTO_POLPI PROTO_POLPI] Potent antimicrobial peptide that acts by disrupting bacterial membrane (PubMed:21745529, PubMed:22450985). Is active against both Gram-positive and Gram-negative bacteria (B.subtilis (MIC=15 ug/ml or 4-16 uM), S.epidermidis (MIC=8-16 uM), S.aureus (MIC=15 ug/ml or 4 uM), E.coli (MIC=8-64 uM) and P.aeruginosa (MIC=64-128 uM)) (PubMed:16129513, PubMed:22450985, PubMed:23836163, PubMed:30534613). Also shows cytotoxicity towards HEK293 cells (32 uM) (PubMed:30534613). Adopts an amphipathic alpha helical conformation, that may allow to partition into the target membrane (PubMed:21745529, PubMed:23836163). Also acts in inflammation since it is chemotactic for polymorphonucleated leukocytes (PMNL) (PubMed:16129513). Shows potent antitumor activity against prostate (PC-3) and bladder (Biu-87) cancer cell lines (PubMed:21745529). Causes a reduced hemolysis to mammalian erythrocytes (HC(50)=50 uM) and has no mast cell degranulation activity at physiological concentrations (PubMed:16129513, PubMed:30534613). In addition, when tested in vitro on the parasite Trypanosoma cruzi (responsible of the Chagas disease), is able to reduce the number of the three forms (epimastigote, trypomastigote and amastigote), probably acting through the apoptotic cell death pathway (PubMed:32360153).<ref>PMID:16129513</ref> <ref>PMID:21745529</ref> <ref>PMID:22450985</ref> <ref>PMID:23836163</ref> <ref>PMID:30534613</ref> <ref>PMID:32360153</ref> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Polybia paulista]] | |||
[[Category: Bloch Y]] | |||
[[Category: Golubev A]] | |||
[[Category: Landau M]] | |||
