2cii: Difference between revisions
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==Overview== | ==Overview== | ||
In the absence of bound peptide ligands, major histocompatibility complex, (MHC) class I molecules are unstable. In an attempt to determine the, minimum requirement for peptide-dependent MHC class I stabilization, we, have used short synthetic peptides derived from the Sendai virus, nucleoprotein epitope (residues 324-332, 1FAPGNYPAL9) to promote its, folding in vitro of H-2D(b). We found that H-2D(b) can be stabilized by, the pentapeptide 5NYPAL9, which is equivalent to the C-terminal portion of, the optimal nonapeptide and includes both the P5 and P9 anchor residues., We have crystallized the complex of the H-2D(b) molecule with the pentamer, and determined the structure to show how a quasi-stable MHC class I, molecule can be formed by occupancy of a single binding pocket in the, peptide-binding groove. | In the absence of bound peptide ligands, major histocompatibility complex, (MHC) class I molecules are unstable. In an attempt to determine the, minimum requirement for peptide-dependent MHC class I stabilization, we, have used short synthetic peptides derived from the Sendai virus, nucleoprotein epitope (residues 324-332, 1FAPGNYPAL9) to promote its, folding in vitro of H-2D(b). We found that H-2D(b) can be stabilized by, the pentapeptide 5NYPAL9, which is equivalent to the C-terminal portion of, the optimal nonapeptide and includes both the P5 and P9 anchor residues., We have crystallized the complex of the H-2D(b) molecule with the pentamer, and determined the structure to show how a quasi-stable MHC class I, molecule can be formed by occupancy of a single binding pocket in the, peptide-binding groove. | ||
==Disease== | |||
Known disease associated with this structure: Hypoproteinemia, hypercatabolic OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=109700 109700]] | |||
==About this Structure== | ==About this Structure== | ||
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[[Category: transmembrane]] | [[Category: transmembrane]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 21:15:49 2007'' | ||
