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'''Correction of X-ray Intensities from an HslV-HslU co-crystal containing lattice translocation defects''' | '''Correction of X-ray Intensities from an HslV-HslU co-crystal containing lattice translocation defects''' | ||
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[[Category: Rho, S H.]] | [[Category: Rho, S H.]] | ||
[[Category: Wang, J.]] | [[Category: Wang, J.]] | ||
[[Category: | [[Category: Atp-dependent proteolysis]] | ||
[[Category: | [[Category: Hslv-hslu]] | ||
[[Category: | [[Category: Lattice translocation defect]] | ||
[[Category: | [[Category: Quaternary structure]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:57:38 2008'' | |||
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Revision as of 13:57, 3 May 2008
Correction of X-ray Intensities from an HslV-HslU co-crystal containing lattice translocation defects
Overview
Because of lattice-translocation defects, two identical but translated lattices can coexist as a single coherent mosaic block in a crystal. The observed structure in such cases is a weighted sum of two identical but translated structures, one from each lattice; the observed structure factors are a weighted vector sum of the structure factors with identical unit amplitudes but shifted phases. The correction of X-ray intensities from a single crystal containing these defects of the hybrid HslV-HslU complex, which consists of Escherichia coli HslU and Bacillus subtilis HslV (also known as CodW), is reported. When intensities are not corrected, a biologically irrelevant complex (with CodW from one lattice and HslU from another) is implied to exist. Only upon correction does a biologically functional CodW-HslU complex structure emerge.
About this Structure
1YYF is a Protein complex structure of sequences from Bacillus subtilis and Escherichia coli. The following page contains interesting information on the relation of 1YYF with [AAA+ Proteases]. Full crystallographic information is available from OCA.
Reference
Correction of X-ray intensities from an HslV-HslU co-crystal containing lattice-translocation defects., Wang J, Rho SH, Park HH, Eom SH, Acta Crystallogr D Biol Crystallogr. 2005 Jul;61(Pt 7):932-41. Epub 2005, Jun 24. PMID:15983416 Page seeded by OCA on Sat May 3 16:57:38 2008
