2ac1: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2ac1.gif|left|200px]] | [[Image:2ac1.gif|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_2ac1", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |||
or leave the SCENE parameter empty for the default display. | |||
| | --> | ||
| | {{STRUCTURE_2ac1| PDB=2ac1 | SCENE= }} | ||
}} | |||
'''Crystal structure of a cell-wall invertase from Arabidopsis thaliana''' | '''Crystal structure of a cell-wall invertase from Arabidopsis thaliana''' | ||
| Line 32: | Line 29: | ||
[[Category: Roy, K Le.]] | [[Category: Roy, K Le.]] | ||
[[Category: Verhaest, M.]] | [[Category: Verhaest, M.]] | ||
[[Category: | [[Category: Five fold beta propeller]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:51:55 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 15:51, 3 May 2008
Crystal structure of a cell-wall invertase from Arabidopsis thaliana
Overview
Cell-wall invertases play crucial roles during plant development. They hydrolyse sucrose into its fructose and glucose subunits by cleavage of the alpha1-beta2 glycosidic bond. Here, the structure of the Arabidopsis thaliana cell-wall invertase 1 (AtcwINV1; gene accession code At3g13790) is described at a resolution of 2.15 A. The structure comprises an N-terminal fivefold beta-propeller domain followed by a C-terminal domain formed by two beta-sheets. The active site is positioned in the fivefold beta-propeller domain, containing the nucleophile Asp23 and the acid/base catalyst Glu203 of the double-displacement enzymatic reaction. The function of the C-terminal domain remains unknown. Unlike in other GH 32 family enzyme structures known to date, in AtcwINV1 the cleft formed between both domains is blocked by Asn299-linked carbohydrates. A preliminary site-directed mutagenesis experiment (Asn299Asp) removed the glycosyl chain but did not alter the activity profile of the enzyme.
About this Structure
2AC1 is a Single protein structure of sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA.
Reference
X-ray diffraction structure of a cell-wall invertase from Arabidopsis thaliana., Verhaest M, Lammens W, Le Roy K, De Coninck B, De Ranter CJ, Van Laere A, Van den Ende W, Rabijns A, Acta Crystallogr D Biol Crystallogr. 2006 Dec;62(Pt 12):1555-63. Epub 2006, Nov 23. PMID:17139091 Page seeded by OCA on Sat May 3 18:51:55 2008
