2chg: Difference between revisions
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'''REPLICATION FACTOR C DOMAINS 1 AND 2''' | '''REPLICATION FACTOR C DOMAINS 1 AND 2''' | ||
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[[Category: Singleton, M R.]] | [[Category: Singleton, M R.]] | ||
[[Category: Wigley, D B.]] | [[Category: Wigley, D B.]] | ||
[[Category: | [[Category: Aaa+ atpase]] | ||
[[Category: | [[Category: Atp-binding]] | ||
[[Category: | [[Category: Clamp loader]] | ||
[[Category: | [[Category: Dna replication]] | ||
[[Category: | [[Category: Dna-binding protein]] | ||
[[Category: | [[Category: Nucleotide-binding]] | ||
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Revision as of 19:09, 3 May 2008
REPLICATION FACTOR C DOMAINS 1 AND 2
Overview
We have investigated the communication between subunits in replication factor C (RFC) from Archaeoglobus fulgidus. Mutation of the proposed arginine finger in the small subunits results in a complex that can still bind ATP but has impaired clamp-loading activity, a process that normally only requires binding of nucleotide. The small subunit alone forms a hexameric ring that is six-fold symmetric in the absence of ATP. However, this symmetry is broken when the nucleotide is bound to the complex. A conformational change associated with nucleotide binding may relate to the opening of PCNA rings by RFC during the loading reaction. The structures also reveal the importance of the N-terminal helix of each subunit at the ATP-binding site. Analysis of mutant protein complexes containing subunits lacking this N-terminal helix reveals key distinct regulatory roles during clamp loading that are different for the large and small subunits in the RFC complex.
About this Structure
2CHG is a Single protein structure of sequence from Archaeoglobus fulgidus. Full crystallographic information is available from OCA.
Reference
Communication between subunits within an archaeal clamp-loader complex., Seybert A, Singleton MR, Cook N, Hall DR, Wigley DB, EMBO J. 2006 May 17;25(10):2209-18. Epub 2006 Apr 20. PMID:16628222 Page seeded by OCA on Sat May 3 22:09:02 2008
