2cjs: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
Line 1: Line 1:
[[Image:2cjs.gif|left|200px]]
[[Image:2cjs.gif|left|200px]]


{{Structure
<!--
|PDB= 2cjs |SIZE=350|CAPTION= <scene name='initialview01'>2cjs</scene>, resolution 1.78&Aring;
The line below this paragraph, containing "STRUCTURE_2cjs", creates the "Structure Box" on the page.
|SITE= <scene name='pdbsite=AC1:Gol+Binding+Site+For+Chain+A'>AC1</scene>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
|LIGAND= <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
|ACTIVITY=  
or leave the SCENE parameter empty for the default display.
|GENE=  
-->
|DOMAIN=
{{STRUCTURE_2cjs| PDB=2cjs  | SCENE= }}  
|RELATEDENTRY=
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cjs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cjs OCA], [http://www.ebi.ac.uk/pdbsum/2cjs PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2cjs RCSB]</span>
}}


'''STRUCTURAL BASIS FOR A MUNC13-1 HOMODIMER- MUNC13-1- RIM HETERODIMER SWITCH: C2-DOMAINS AS VERSATILE PROTEIN-PROTEIN INTERACTION MODULES'''
'''STRUCTURAL BASIS FOR A MUNC13-1 HOMODIMER- MUNC13-1- RIM HETERODIMER SWITCH: C2-DOMAINS AS VERSATILE PROTEIN-PROTEIN INTERACTION MODULES'''
Line 32: Line 29:
[[Category: Sudhof, T C.]]
[[Category: Sudhof, T C.]]
[[Category: Tomchick, D R.]]
[[Category: Tomchick, D R.]]
[[Category: alternative splicing]]
[[Category: Alternative splicing]]
[[Category: c2 domain]]
[[Category: C2 domain]]
[[Category: coiled coil]]
[[Category: Coiled coil]]
[[Category: exocytosis]]
[[Category: Exocytosis]]
[[Category: metal-binding]]
[[Category: Metal-binding]]
[[Category: munc13]]
[[Category: Munc13]]
[[Category: neurotransmitter release]]
[[Category: Neurotransmitter release]]
[[Category: neurotransmitter transport]]
[[Category: Neurotransmitter transport]]
[[Category: phorbol-ester binding]]
[[Category: Phorbol-ester binding]]
[[Category: protein-protein interaction]]
[[Category: Protein-protein interaction]]
[[Category: rim]]
[[Category: Rim]]
[[Category: synapse]]
[[Category: Synapse]]
[[Category: synaptosome]]
[[Category: Synaptosome]]
[[Category: transport]]
[[Category: Transport]]
[[Category: zinc]]
[[Category: Zinc]]
[[Category: zinc finger]]
[[Category: Zinc finger]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 22:18:46 2008''
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:22:30 2008''

Revision as of 19:18, 3 May 2008

File:2cjs.gif

Template:STRUCTURE 2cjs

STRUCTURAL BASIS FOR A MUNC13-1 HOMODIMER- MUNC13-1- RIM HETERODIMER SWITCH: C2-DOMAINS AS VERSATILE PROTEIN-PROTEIN INTERACTION MODULES


Overview

C(2) domains are well characterized as Ca(2+)/phospholipid-binding modules, but little is known about how they mediate protein-protein interactions. In neurons, a Munc13-1 C(2)A-domain/RIM zinc-finger domain (ZF) heterodimer couples synaptic vesicle priming to presynaptic plasticity. We now show that the Munc13-1 C(2)A domain homodimerizes, and that homodimerization competes with Munc13-1/RIM heterodimerization. X-ray diffraction studies guided by nuclear magnetic resonance (NMR) experiments reveal the crystal structures of the Munc13-1 C(2)A-domain homodimer and the Munc13-1 C(2)A-domain/RIM ZF heterodimer at 1.44 A and 1.78 A resolution, respectively. The C(2)A domain adopts a beta-sandwich structure with a four-stranded concave side that mediates homodimerization, leading to the formation of an eight-stranded beta-barrel. In contrast, heterodimerization involves the bottom tip of the C(2)A-domain beta-sandwich and a C-terminal alpha-helical extension, which wrap around the RIM ZF domain. Our results describe the structural basis for a Munc13-1 homodimer-Munc13-1/RIM heterodimer switch that may be crucial for vesicle priming and presynaptic plasticity, uncovering at the same time an unexpected versatility of C(2) domains as protein-protein interaction modules, and illustrating the power of combining NMR spectroscopy and X-ray crystallography to study protein complexes.

About this Structure

2CJS is a Protein complex structure of sequences from Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

Structural basis for a Munc13-1 homodimer to Munc13-1/RIM heterodimer switch., Lu J, Machius M, Dulubova I, Dai H, Sudhof TC, Tomchick DR, Rizo J, PLoS Biol. 2006 Jul;4(7):e192. PMID:16732694 Page seeded by OCA on Sat May 3 22:18:46 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=2cjs&oldid=469414"