2czt: Difference between revisions
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'''lipocalin-type prostaglandin D synthase''' | '''lipocalin-type prostaglandin D synthase''' | ||
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[[Category: Urade, Y.]] | [[Category: Urade, Y.]] | ||
[[Category: Yamamoto, M.]] | [[Category: Yamamoto, M.]] | ||
[[Category: | [[Category: C2221 native]] | ||
[[Category: | [[Category: Lipocalin]] | ||
[[Category: | [[Category: Riken structural genomics/proteomics initiative]] | ||
[[Category: | [[Category: Rsgi]] | ||
[[Category: | [[Category: Structural genomic]] | ||
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Revision as of 20:25, 3 May 2008
lipocalin-type prostaglandin D synthase
Overview
Lipocalin-type prostaglandin D synthase is the key enzyme for the production of prostaglandin D(2), a potent endogenous somnogen, in the brain. We cloned, produced, and crystallized the native enzyme and selenomethionyl Cys(65)Ala mutants of the recombinant mouse protein by the hanging drop vapor-diffusion method with both malonate and citrate as precipitants. The native crystals obtained with malonate belong to orthorhombic space group P2(1)2(1)2(1) with lattice constants a = 46.2, b = 66.8, and c = 105.3 A. The selenomethionyl crystals obtained with citrate belong to orthorhombic space group C222(1) with lattice constants a = 45.5, b = 66.8, and c = 104.5 A. The native crystals diffracted beyond 2.1 A resolution.
About this Structure
2CZT is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Cloning, expression, crystallization, and preliminary X-ray analysis of recombinant mouse lipocalin-type prostaglandin D synthase, a somnogen-producing enzyme., Irikura D, Kumasaka T, Yamamoto M, Ago H, Miyano M, Kubata KB, Sakai H, Hayaishi O, Urade Y, J Biochem. 2003 Jan;133(1):29-32. PMID:12761195 Page seeded by OCA on Sat May 3 23:25:52 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Mus musculus
- Prostaglandin-D synthase
- Single protein
- Ago, H.
- Aritake, K.
- Hayaishi, O.
- Inoue, T.
- Irikura, D.
- Kumasaka, T.
- Miyano, M.
- RSGI, RIKEN Structural Genomics/Proteomics Initiative.
- Urade, Y.
- Yamamoto, M.
- C2221 native
- Lipocalin
- Riken structural genomics/proteomics initiative
- Rsgi
- Structural genomic
