2gov: Difference between revisions
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'''Solution structure of Murine p22HBP''' | '''Solution structure of Murine p22HBP''' | ||
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[[Category: Peterson, F C.]] | [[Category: Peterson, F C.]] | ||
[[Category: Volkman, B F.]] | [[Category: Volkman, B F.]] | ||
[[Category: | [[Category: Center for eukaryotic structural genomic]] | ||
[[Category: | [[Category: Cesg]] | ||
[[Category: | [[Category: Heme binding]] | ||
[[Category: | [[Category: P22hbp]] | ||
[[Category: | [[Category: Protein structure initiative]] | ||
[[Category: | [[Category: Psi]] | ||
[[Category: | [[Category: Structural genomic]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:20:53 2008'' | |||
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Revision as of 02:20, 4 May 2008
Solution structure of Murine p22HBP
Overview
Murine p22HBP, a 22-kDa monomer originally identified as a cytosolic heme-binding protein ubiquitously expressed in various tissues, has 27% sequence identity to murine SOUL, a heme-binding hexamer specifically expressed in the retina. In contrast to murine SOUL, which binds one heme per subunit via coordination of the Fe(III)-heme to a histidine, murine p22HBP binds one heme molecule per subunit with no specific axial ligand coordination of the Fe(III)-heme. Using intrinsic protein fluorescence quenching, the values for the dissociation constants of p22HBP for hemin and protoporphyrin-IX were determined to be in the low nanomolar range. The three-dimensional structure of murine p22HBP, the first for a protein from the SOUL/HBP family, was determined by NMR methods to consist of a 9-stranded distorted beta-barrel flanked by two long alpha-helices. Although homologous domains have been found in three bacterial proteins, two of which are transcription factors, the fold determined for p22HBP corresponds to a novel alpha plus beta fold in a eukaryotic protein. Chemical shift mapping localized the tetrapyrrole binding site to a hydrophobic cleft formed by residues from helix alphaA and an extended loop. In an attempt to assess the structural basis for tetrapyrrole binding in the SOUL/HBP family, models for the p22HBP-protoporphyrin-IX complex and the SOUL protein were generated by manual docking and automated methods.
About this Structure
2GOV is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
The first structure from the SOUL/HBP family of heme-binding proteins, murine P22HBP., Dias JS, Macedo AL, Ferreira GC, Peterson FC, Volkman BF, Goodfellow BJ, J Biol Chem. 2006 Oct 20;281(42):31553-61. Epub 2006 Aug 10. PMID:16905545 Page seeded by OCA on Sun May 4 05:20:53 2008
