2gv5: Difference between revisions

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[[Image:2gv5.gif|left|200px]]
[[Image:2gv5.gif|left|200px]]


{{Structure
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|GENE= CDC31 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), SFI1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
-->
|DOMAIN=
{{STRUCTURE_2gv5| PDB=2gv5  | SCENE= }}  
|RELATEDENTRY=
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gv5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gv5 OCA], [http://www.ebi.ac.uk/pdbsum/2gv5 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2gv5 RCSB]</span>
}}


'''crystal structure of Sfi1p/Cdc31p complex'''
'''crystal structure of Sfi1p/Cdc31p complex'''
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[[Category: Sandercock, A M.]]
[[Category: Sandercock, A M.]]
[[Category: Williams, R L.]]
[[Category: Williams, R L.]]
[[Category: cdc31p]]
[[Category: Cdc31p]]
[[Category: centrin]]
[[Category: Centrin]]
[[Category: centrosome]]
[[Category: Centrosome]]
[[Category: sfi1p]]
[[Category: Sfi1p]]
[[Category: spindle pole body]]
[[Category: Spindle pole body]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 05:34:36 2008''
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:22:02 2008''

Revision as of 02:34, 4 May 2008

File:2gv5.gif

Template:STRUCTURE 2gv5

crystal structure of Sfi1p/Cdc31p complex


Overview

Centrins are calmodulin-like proteins present in centrosomes and yeast spindle pole bodies (SPBs) and have essential functions in their duplication. The Saccharomyces cerevisiae centrin, Cdc31p, binds Sfi1p on multiple conserved repeats; both proteins localize to the SPB half-bridge, where the new SPB is assembled. The crystal structures of Sfi1p-centrin complexes containing several repeats show Sfi1p as an alpha helix with centrins wrapped around each repeat and similar centrin-centrin contacts between each repeat. Electron microscopy (EM) shadowing of an Sfi1p-centrin complex with 15 Sfi1 repeats and 15 centrins bound showed filaments 60 nm long, compatible with all the Sfi1 repeats as a continuous alpha helix. Immuno-EM localization of the Sfi1p N and C termini showed Sfi1p-centrin filaments spanning the length of the half-bridge with the Sfi1p N terminus at the SPB. This suggests a model for SPB duplication where the half-bridge doubles in length by association of the Sfi1p C termini, thereby providing a new Sfi1p N terminus to initiate SPB assembly.

About this Structure

2GV5 is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Structural role of Sfi1p-centrin filaments in budding yeast spindle pole body duplication., Li S, Sandercock AM, Conduit P, Robinson CV, Williams RL, Kilmartin JV, J Cell Biol. 2006 Jun 19;173(6):867-77. PMID:16785321 Page seeded by OCA on Sun May 4 05:34:36 2008

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