2iu1: Difference between revisions
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{{STRUCTURE_2iu1| PDB=2iu1 | SCENE= }} | |||
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'''CRYSTAL STRUCTURE OF EIF5 C-TERMINAL DOMAIN''' | '''CRYSTAL STRUCTURE OF EIF5 C-TERMINAL DOMAIN''' | ||
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[[Category: Bieniossek, C.]] | [[Category: Bieniossek, C.]] | ||
[[Category: Schuetz, P.]] | [[Category: Schuetz, P.]] | ||
[[Category: | [[Category: Eif5]] | ||
[[Category: | [[Category: Gtp-binding]] | ||
[[Category: | [[Category: Initiation factor]] | ||
[[Category: | [[Category: Mfc]] | ||
[[Category: | [[Category: Nucleotide-binding]] | ||
[[Category: | [[Category: Phosphorylation]] | ||
[[Category: | [[Category: Protein biosynthesis]] | ||
[[Category: | [[Category: Transcription]] | ||
[[Category: | [[Category: Translation inititation]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:52:40 2008'' | |||
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Revision as of 04:52, 4 May 2008
CRYSTAL STRUCTURE OF EIF5 C-TERMINAL DOMAIN
Overview
The carboxy-terminal domain (CTD) of eukaryotic initiation factor 5 (eIF5) plays a central role in the formation of the multifactor complex (MFC), an important intermediate for the 43 S pre-initiation complex assembly. The IF5-CTD interacts directly with the translation initiation factors eIF1, eIF2-beta, and eIF3c, thus forming together with eIF2 bound Met-tRNA(i)(Met) the MFC. In this work we present the high resolution crystal structure of eIF5-CTD. This domain of the protein is exclusively composed out of alpha-helices and is homologous to the carboxy-terminal domain of eIF2B-epsilon (eIF2Bepsilon-CTD). The most striking difference in the two structures is an additional carboxy-terminal helix in eIF5. The binding sites of eIF2-beta, eIF3 and eIF1 were mapped onto the structure. eIF2-beta and eIF3 bind to non-overlapping patches of negative and positive electrostatic potential, respectively.
About this Structure
2IU1 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The crystal structure of the carboxy-terminal domain of human translation initiation factor eIF5., Bieniossek C, Schutz P, Bumann M, Limacher A, Uson I, Baumann U, J Mol Biol. 2006 Jul 7;360(2):457-65. Epub 2006 May 24. PMID:16781736 Page seeded by OCA on Sun May 4 07:52:40 2008
