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'''X-RAY STRUCTURE OF THE CHAPERONE PAPD IN COMPLEX WITH THE PILUS TERMINATOR SUBUNIT PAPH AT 2.3 ANGSTROM RESOLUTION''' | '''X-RAY STRUCTURE OF THE CHAPERONE PAPD IN COMPLEX WITH THE PILUS TERMINATOR SUBUNIT PAPH AT 2.3 ANGSTROM RESOLUTION''' | ||
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[[Category: Verger, D.]] | [[Category: Verger, D.]] | ||
[[Category: Waksman, G.]] | [[Category: Waksman, G.]] | ||
[[Category: | [[Category: Chaperone]] | ||
[[Category: | [[Category: Chaperone/ surface active protein complex]] | ||
[[Category: | [[Category: Fimbria]] | ||
[[Category: | [[Category: Immunoglobulin domain]] | ||
[[Category: | [[Category: P5 pocket]] | ||
[[Category: | [[Category: Papd]] | ||
[[Category: | [[Category: Paph]] | ||
[[Category: | [[Category: Periplasmic]] | ||
[[Category: | [[Category: Pilus termination]] | ||
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Revision as of 05:15, 4 May 2008
X-RAY STRUCTURE OF THE CHAPERONE PAPD IN COMPLEX WITH THE PILUS TERMINATOR SUBUNIT PAPH AT 2.3 ANGSTROM RESOLUTION
Overview
P pili are important adhesive fibres that are assembled by the conserved chaperone-usher pathway. During pilus assembly, the subunits are incorporated into the growing fibre by the donor-strand exchange mechanism, whereby the beta-strand of the chaperone, which complements the incomplete immunoglobulin fold of each subunit, is displaced by the amino-terminal extension of an incoming subunit in a zip-in-zip-out exchange process that is initiated at the P5 pocket, an exposed hydrophobic pocket in the groove of the subunit. In vivo, termination of P pilus growth requires a specialized subunit, PapH. Here, we show that PapH is incorporated at the base of the growing pilus, where it is unable to undergo donor-strand exchange. This inability is not due to a stronger PapD-PapH interaction, but to a lack of a P5 initiator pocket in the PapH structure, suggesting that PapH terminates pilus growth because it is lacking the initiation point by which donor-strand exchange proceeds.
About this Structure
2J2Z is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Molecular mechanism of P pilus termination in uropathogenic Escherichia coli., Verger D, Miller E, Remaut H, Waksman G, Hultgren S, EMBO Rep. 2006 Dec;7(12):1228-32. Epub 2006 Nov 3. PMID:17082819 Page seeded by OCA on Sun May 4 08:15:43 2008
