2j52: Difference between revisions
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{{STRUCTURE_2j52| PDB=2j52 | SCENE= }} | |||
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'''SOLUTION STRUCTURE OF GB1 DOMAIN PROTEIN G AND LOW AND HIGH PRESSURE.''' | '''SOLUTION STRUCTURE OF GB1 DOMAIN PROTEIN G AND LOW AND HIGH PRESSURE.''' | ||
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[[Category: Williamson, M P.]] | [[Category: Williamson, M P.]] | ||
[[Category: Wilton, D J.]] | [[Category: Wilton, D J.]] | ||
[[Category: | [[Category: Cell wall]] | ||
[[Category: | [[Category: Igg-binding protein]] | ||
[[Category: | [[Category: Immunoglobulin]] | ||
[[Category: | [[Category: Peptidoglycan-anchor]] | ||
[[Category: | [[Category: Pressure]] | ||
[[Category: | [[Category: Protein g]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 08:20:51 2008'' | |||
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Revision as of 05:20, 4 May 2008
SOLUTION STRUCTURE OF GB1 DOMAIN PROTEIN G AND LOW AND HIGH PRESSURE.
Overview
The solution structure of the GB1 domain of protein G at a pressure of 2 kbar is presented. The structure was calculated as a change from an energy-minimised low-pressure structure using (1)H chemical shifts. Two separate changes can be characterised: a compression/distortion, which is linear with pressure; and a stabilisation of an alternative folded state. On application of pressure, linear chemical shift changes reveal that the backbone structure changes by about 0.2 A root mean square, and is compressed by about 1% overall. The alpha-helix compresses, particularly at the C-terminal end, and moves toward the beta-sheet, while the beta-sheet is twisted, with the corners closest to the alpha-helix curling up towards it. The largest changes in structure are along the second beta-strand, which becomes more twisted. This strand is where the protein binds to IgG. Curved chemical shift changes with pressure indicate that high pressure also populates an alternative structure with a distortion towards the C-terminal end of the helix, which is likely to be caused by insertion of a water molecule. Proteins 2007. (c) 2007 Wiley-Liss, Inc.
About this Structure
2J52 is a Single protein structure of sequence from Streptococcus sp.. Full crystallographic information is available from OCA.
Reference
Pressure-induced changes in the solution structure of the GB1 domain of protein G., Wilton DJ, Tunnicliffe RB, Kamatari YO, Akasaka K, Williamson MP, Proteins. 2007 Dec 12;. PMID:18076052 Page seeded by OCA on Sun May 4 08:20:51 2008
