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'''NMR solution structure of Blo t 5, a major mite allergen from Blomia tropicalis''' | '''NMR solution structure of Blo t 5, a major mite allergen from Blomia tropicalis''' | ||
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[[Category: Kuo, I.]] | [[Category: Kuo, I.]] | ||
[[Category: Naik, M T.]] | [[Category: Naik, M T.]] | ||
[[Category: | [[Category: Allergen]] | ||
[[Category: | [[Category: Blo t 5]] | ||
[[Category: | [[Category: Blomia tropicali]] | ||
[[Category: | [[Category: Dust mite]] | ||
[[Category: | [[Category: Group 5]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:01:55 2008'' | |||
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Revision as of 06:01, 4 May 2008
NMR solution structure of Blo t 5, a major mite allergen from Blomia tropicalis
Overview
Blo t 5 is the major allergen from Blomia tropicalis mites and shows strong IgE reactivity with up to 90% of asthmatic and rhinitis patients' sera. The NMR solution structure of Blo t 5 comprises three long alpha helices, forming a coiled-coil, triple-helical bundle with a left-handed twist. TROSY-NMR experiments were used to study Blo t 5 interaction with the Fab' of a specific monoclonal antibody, mAb 4A7. The mAb epitope comprises two closely spaced surfaces, I and II, connected by a sharp turn and bearing critical residues Asn46 and Lys47 on one surface, and Lys54 and Arg57 on the other. This discontinuous epitope overlaps with the human IgE epitope(s) of Blo t 5. Epitope surface II is further predicted to undergo conformational exchange in the millisecond to microsecond range. The results presented are critical for the design of a hypoallergenic Blo t 5 for efficacious immunotherapy of allergic diseases.
About this Structure
2JMH is a Single protein structure of sequence from Blomia tropicalis. Full crystallographic information is available from OCA.
Reference
Roles of structure and structural dynamics in the antibody recognition of the allergen proteins: an NMR study on Blomia tropicalis major allergen., Naik MT, Chang CF, Kuo IC, Kung CC, Yi FC, Chua KY, Huang TH, Structure. 2008 Jan;16(1):125-36. PMID:18184590 Page seeded by OCA on Sun May 4 09:01:55 2008
