2oiu: Difference between revisions
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'''L1 Ribozyme Ligase circular adduct''' | '''L1 Ribozyme Ligase circular adduct''' | ||
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==About this Structure== | ==About this Structure== | ||
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OIU OCA]. | |||
==Reference== | ==Reference== | ||
The structural basis of ribozyme-catalyzed RNA assembly., Robertson MP, Scott WG, Science. 2007 Mar 16;315(5818):1549-53. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17363667 17363667] | The structural basis of ribozyme-catalyzed RNA assembly., Robertson MP, Scott WG, Science. 2007 Mar 16;315(5818):1549-53. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17363667 17363667] | ||
[[Category: Robertson, M P]] | [[Category: Robertson, M P]] | ||
[[Category: Scott, W G.]] | [[Category: Scott, W G.]] | ||
[[Category: | [[Category: Active site]] | ||
[[Category: | [[Category: In vitro selection]] | ||
[[Category: | [[Category: Ligase]] | ||
[[Category: | [[Category: Ribozyme]] | ||
[[Category: | [[Category: Rna]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:00:21 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 08:00, 4 May 2008
L1 Ribozyme Ligase circular adduct
Overview
Life originated, according to the RNA World hypothesis, from self-replicating ribozymes that catalyzed ligation of RNA fragments. We have solved the 2.6 angstrom crystal structure of a ligase ribozyme that catalyzes regiospecific formation of a 5' to 3' phosphodiester bond between the 5'-triphosphate and the 3'-hydroxyl termini of two RNA fragments. Invariant residues form tertiary contacts that stabilize a flexible stem of the ribozyme at the ligation site, where an essential magnesium ion coordinates three phosphates. The structure of the active site permits us to suggest how transition-state stabilization and a general base may catalyze the ligation reaction required for prebiotic RNA assembly.
About this Structure
Full crystallographic information is available from OCA.
Reference
The structural basis of ribozyme-catalyzed RNA assembly., Robertson MP, Scott WG, Science. 2007 Mar 16;315(5818):1549-53. PMID:17363667 Page seeded by OCA on Sun May 4 11:00:21 2008
