2p73: Difference between revisions
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{{STRUCTURE_2p73| PDB=2p73 | SCENE= }} | |||
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'''crystal structure of a glycosyltransferase involved in the glycosylation of the major capsid of PBCV-1''' | '''crystal structure of a glycosyltransferase involved in the glycosylation of the major capsid of PBCV-1''' | ||
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[[Category: Xiang, Y.]] | [[Category: Xiang, Y.]] | ||
[[Category: Zhang, Y.]] | [[Category: Zhang, Y.]] | ||
[[Category: | [[Category: Crystal structure]] | ||
[[Category: | [[Category: Glycosyltransferase]] | ||
[[Category: | [[Category: Pbcv-1 a64r]] | ||
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Revision as of 09:31, 4 May 2008
crystal structure of a glycosyltransferase involved in the glycosylation of the major capsid of PBCV-1
Overview
Paramecium bursaria chlorella virus-1 encodes at least five putative glycosyltransferases that are probably involved in the synthesis of the glycan components of the viral major capsid protein. The 1.6 A crystal structure of one of these glycosyltransferases (A64R) has a mixed alpha/beta fold containing a central, six-stranded beta sheet flanked by alpha helices. Crystal structures of A64R, complexed with UDP, CMP, or GDP, established that only UDP bound to A64R in the presence of Mn(2+), consistent with its high structural similarity to glycosyltransferases which utilize UDP as the sugar carrier. The structure of the complex of A64R, UDP-glucose, and Mn(2+) showed that the largest conformational change occurred when hydrogen bonds were formed with the ligands. Unlike UDP-glucose, UDP-galactose and UDP-GlcNAc did not bind to A64R, suggesting a selective binding of UDP-glucose. Thus, UDP-glucose is most likely the sugar donor for A64R, consistent with glucose occurring in the virus major capsid protein glycans.
About this Structure
2P73 is a Single protein structure of sequence from Paramecium bursaria chlorella virus 1. Full crystallographic information is available from OCA.
Reference
Structure and function of a chlorella virus-encoded glycosyltransferase., Zhang Y, Xiang Y, Van Etten JL, Rossmann MG, Structure. 2007 Sep;15(9):1031-9. PMID:17850743 Page seeded by OCA on Sun May 4 12:31:24 2008
