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'''Structure of the Pho85-Pho80 CDK-cyclin Complex of the Phosphate-responsive Signal Transduction Pathway with Bound ATP-gamma-S''' | '''Structure of the Pho85-Pho80 CDK-cyclin Complex of the Phosphate-responsive Signal Transduction Pathway with Bound ATP-gamma-S''' | ||
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[[Category: Shea, E K.O.]] | [[Category: Shea, E K.O.]] | ||
[[Category: Zhang, W.]] | [[Category: Zhang, W.]] | ||
[[Category: | [[Category: Cyclin]] | ||
[[Category: | [[Category: Cyclin-dependent kinase]] | ||
[[Category: | [[Category: Signaling protein,transferase/cell cycle complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 13:25:01 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 10:25, 4 May 2008
Structure of the Pho85-Pho80 CDK-cyclin Complex of the Phosphate-responsive Signal Transduction Pathway with Bound ATP-gamma-S
Overview
The ability to sense and respond appropriately to environmental changes is a primary requirement of all living organisms. In response to phosphate limitation, Saccharomyces cerevisiae induces transcription of a set of genes involved in the regulation of phosphate acquisition from the ambient environment. A signal transduction pathway (the PHO pathway) mediates this response, with Pho85-Pho80 playing a vital role. Here we report the X-ray structure of Pho85-Pho80, a prototypic structure of a CDK-cyclin complex functioning in transcriptional regulation in response to environmental changes. The structure revealed a specific salt link between a Pho85 arginine and a Pho80 aspartate that makes phosphorylation of the Pho85 activation loop dispensable and that maintains a Pho80 loop conformation for possible substrate recognition. It further showed two sites on the Pho80 cyclin for high-affinity binding of the transcription factor substrate (Pho4) and the CDK inhibitor (Pho81) that are markedly distant to each other and the active site.
About this Structure
2PMI is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structure of the Pho85-Pho80 CDK-cyclin complex of the phosphate-responsive signal transduction pathway., Huang K, Ferrin-O'Connell I, Zhang W, Leonard GA, O'Shea EK, Quiocho FA, Mol Cell. 2007 Nov 30;28(4):614-23. PMID:18042456 Page seeded by OCA on Sun May 4 13:25:01 2008
