2r0g: Difference between revisions
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{{STRUCTURE_2r0g| PDB=2r0g | SCENE= }} | |||
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'''Chromopyrrolic acid-soaked RebC with bound 7-carboxy-K252c''' | '''Chromopyrrolic acid-soaked RebC with bound 7-carboxy-K252c''' | ||
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[[Category: Ryan, K S.]] | [[Category: Ryan, K S.]] | ||
[[Category: 7-carboxy-k252c]] | [[Category: 7-carboxy-k252c]] | ||
[[Category: | [[Category: Chromopyrrolic acid]] | ||
[[Category: | [[Category: Flavin adenine dinucleotide]] | ||
[[Category: | [[Category: Monooxygenase]] | ||
[[Category: | [[Category: Oxidoreductase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 16:01:20 2008'' | |||
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Revision as of 13:01, 4 May 2008
Chromopyrrolic acid-soaked RebC with bound 7-carboxy-K252c
Overview
The biosynthesis of rebeccamycin, an antitumor compound, involves the remarkable eight-electron oxidation of chlorinated chromopyrrolic acid. Although one rebeccamycin biosynthetic enzyme is capable of generating low levels of the eight-electron oxidation product on its own, a second protein, RebC, is required to accelerate product formation and eliminate side reactions. However, the mode of action of RebC was largely unknown. Using crystallography, we have determined a likely function for RebC as a flavin hydroxylase, captured two snapshots of its dynamic catalytic cycle, and trapped a reactive molecule, a putative substrate, in its binding pocket. These studies strongly suggest that the role of RebC is to sequester a reactive intermediate produced by its partner protein and to react with it enzymatically, preventing its conversion to a suite of degradation products that includes, at low levels, the desired product.
About this Structure
2R0G is a Single protein structure of sequence from Lechevalieria aerocolonigenes. Full crystallographic information is available from OCA.
Reference
Crystallographic trapping in the rebeccamycin biosynthetic enzyme RebC., Ryan KS, Howard-Jones AR, Hamill MJ, Elliott SJ, Walsh CT, Drennan CL, Proc Natl Acad Sci U S A. 2007 Sep 25;104(39):15311-6. Epub 2007 Sep 14. PMID:17873060 Page seeded by OCA on Sun May 4 16:01:20 2008
